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Detection of genetic variation among single-spore isolates and identification of genets of Armillaria ostoyae by AFLP analysis with Texas RedTM-labeled selective primer


Detection of genetic variation among single-spore isolates and identification of genets of Armillaria ostoyae by AFLP analysis with Texas RedTM-labeled selective primer



Mycoscience 42(1): 123-127



ISSN/ISBN: 1340-3540

DOI: 10.1007/bf02463985

Amplified fragment length polymorphism (AFLP) analysis was applied to A. ostoyae isolates (single-spore isolates and field isolates from the same forest). For the detection of AFLP, we have developed a modified method using DNA sequencer with Texas Red-labelled selective primer. In the analysis of single-spore isolates, this technique provided large numbers of highly polymorphic DNA markers, which can be used to identify genets.

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Accession: 003704935

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