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Detection of hazelnut DNA traces in chocolate by PCR


Detection of hazelnut DNA traces in chocolate by PCR



International Journal of Food Science and Technology 38(6): 633-640



ISSN/ISBN: 0950-5423

DOI: 10.1046/j.1365-2621.2003.00722.x

By use of the DneasyTM Plant Tissue kit (Qiagen Inc.) plant DNA could be extracted from chocolate and related matrices. The polymerase chain reaction (PCR) detection of mitochondrial plant DNA is directly correlated with the length of the amplified fragment indicating shearing of DNA during chocolate production. Hazelnut DNA could be specifically detected in chocolate matrices with primers derived from the intron between exon B and C of the mitochondrial gene nad1.

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