+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Detection of infectious bronchitis virus by multiplex polymerase chain reaction and sequence analysis



Detection of infectious bronchitis virus by multiplex polymerase chain reaction and sequence analysis



Journal of Virological Methods 109(1): 31-37



A multiplex reverse transcription-polymerase chain reaction (RT-PCR) was developed to amplify the S1 and S2 genes of vaccine and recent Taiwanese isolates of infectious bronchitis virus (IBV). DNA fragments of 228 and 400 base pairs in length were amplified among IBV isolates in multiplex PCR, suggesting that there were no apparent deletions or insertions in these regions. No PCR products were amplified from unrelated avian viruses and negative controls. The results suggested that multiplex PCR provided a specific and sensitive approach for identification of IBV isolates. Sequence analysis of the hypervariable region (HVR) of S1 gene exhibited high variations among Taiwanese IBV isolates. The TWI and TWII groups were about 84-98 and 94-99% identity within the groups. American strains were most divergent sharing only 60% homology with TWI and TWII Taiwanese strains. The Mass group varied 0-10% among each other and had over 70% homology with TWI and TWII Taiwanese strains. A phylogenetic tree based on the nucleotide sequences of the HVR of S1 gene revealed that Taiwanese IBV isolates had evolved into three groups (TWI, TWII, and Mass). This suggested that there were multiple groups of viruses cocirculating in Taiwan.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003705008

Download citation: RISBibTeXText

PMID: 12668265

DOI: 10.1016/s0166-0934(03)00041-7


Related references

Differential detection of turkey coronavirus, infectious bronchitis virus, and bovine coronavirus by a multiplex polymerase chain reaction. Journal of Virological Methods 131(1): 86-91, 2006

The genotyping of infectious bronchitis virus in Taiwan by a multiplex amplification refractory mutation system reverse transcription polymerase chain reaction. Journal of Veterinary Diagnostic Investigation 26(6): 721-733, 2014

Universal oligonucleotides for the detection of infectious bronchitis virus by the polymerase chain reaction. Avian Pathology 25(4): 817-836, 1996

Infectious bronchitis virus detection in allantoic fluid using the polymerase chain reaction and a DNA probe. Avian Diseases 36(2): 403-409, 1992

CDNA probe and reverse transcriptase-polymerase chain reaction for the detection of infectious bronchitis virus. Proceedings of Western Poultry Disease Conference: 4th) 46, 1995

Polymerase chain reaction and a biotin-labeled DNA probe for detection of infectious bronchitis virus in chickens. Avian Diseases 37(1): 149-156, 1993

Detection of infectious bronchitis virus serotypes by reverse transcription polymerase chain reaction in broiler chickens. Springerplus 2(1): 36, 2013

Study on the application of reverse transcription polymerase chain reaction for the detection of the nucleic acid of infectious bronchitis virus. Chinese Journal of Veterinary Medicine 23(1): 5-7, 1997

Multiplex reverse transcriptase-polymerase chain reaction for simultaneous detection of aquatic birnaviruses and infectious hematopoietic necrosis virus (IHNV). 1996

Detection of infectious bronchitis virus by real-time reverse transcriptase-polymerase chain reaction and identification of a quasispecies in the Beaudette strain. Avian diseases 47(3): 718-724, 2003

Microwave or autoclave treatments destroy the infectivity of infectious bronchitis virus and avian pneumovirus but allow detection by reverse transcriptase-polymerase chain reaction. Avian Pathology 33(3): 303-306, 2004

Detection of avian infectious bronchitis virus in infected allantoic fluid using S1 gene serotype specific reverse transcriptase-polymerase chain reaction (RT-PCR). Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases 24(1): 39-42, 2003

Differentiation of infectious bronchitis virus serotypes using polymerase chain reaction and restriction fragment length polymorphism analysis. Avian Diseases 37(1): 194-202, 1993

Sequence analysis of Malaysian infectious bursal disease virus isolate and the use of reverse transcriptase nested polymerase chain reaction enzyme-linked immunosorbent assay for the detection of VP2 hypervariable region. Avian Diseases 47(1): 154-162, 2003

Detection and strain differentiation of infectious bronchitis virus in tracheal tissues from experimentally infected chickens by reverse transcription-polymerase chain reaction. Comparison with an immunohistochemical technique. Avian Pathology 28(4): 327-335, 1999