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Detection of retinol-binding protein in bovine yolk sac, chorion and allantois by immunoperoxidase method


Detection of retinol-binding protein in bovine yolk sac, chorion and allantois by immunoperoxidase method



Asian Australasian Journal of Animal Sciences 15(6): 783-788



ISSN/ISBN: 1011-2367

Bovine yolk sac at day 24 of pregnancy, and placental membranes (chorion and allantois) from days 70 and 100 of pregnancy were isolated and cultured in a modified minimum essential medium in the presence of ethionine. Proteins synthesized and secreted by isolated bovine yolk sac, chorion and allantois were analysed by fluorography of two-dimensional polyacrylamide gel electrophoresis.

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