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Detection of the phylogenetic origin of different populations of Spanish sows by pyrosequencing the region of the cytochrome b gene of pig mitochondrial DNA


Detection of the phylogenetic origin of different populations of Spanish sows by pyrosequencing the region of the cytochrome b gene of pig mitochondrial DNA



ITEA Produccion Animal 96A(3): 220-224



The cytochrome b (Cyt B) region of the mitochondrial DNA (mtDNA) of 234 sows from 5 breeds (Iberian, Majorcan, Landrace, Large White and Pietrain) was analysed. We used the pyrosequencing technique for sequencing this region. This method is based on real-time sequencing and the detection of the bioluminiscence produced when each nucleotide is incorporated in the growing DNA chain. The objective of this experiment was to determine the Asian or European origin of the 5 breeds mentioned above.

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