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Detection of the ryanodine receptor gene mutation associated with porcine stress syndrome from pig hair roots by PCR-RFLP


Detection of the ryanodine receptor gene mutation associated with porcine stress syndrome from pig hair roots by PCR-RFLP



Korean Journal of Veterinary Research 42(1): 65-71



We have utilized the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to detect the ryanodine receptor (RYR1) gene mutation and to estimate the genotype frequencies of the RYR1 gene in the commercial crossbred pig population. The exon region (659 bp) including point mutation (C -> T; Arg -> Cys) in the porcine ryanodine receptor gene, which is a causal mutation for PSS, was amplified by PCR and digested with Cfo I restriction enzyme.

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