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Determination of chondroitin sulfate in nutritional supplements by liquid chromatography


Determination of chondroitin sulfate in nutritional supplements by liquid chromatography



Journal of Liquid Chromatography and Related Technologies 23(18): 2851-2860



ISSN/ISBN: 1082-6076

DOI: 10.1081/jlc-100101237

A size exclusion chromatography (SEC) method for the determination of chondroitin sulfate (CS) in nutritional supplements was developed and validated based on USP guidelines. Samples were dissolved in water and analysed using a Phenomenex BioSep-SEC-S2000 column with an isocratic mobile phase consisting of 0.1 M sodium phosphate at pH=7.0. CS was detected with a photodiode array detector at 207 nm. Data were collected from 200-360 nm.

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