Determination of hypericin and pseudohypericin in extracts from Hypericum perforatum L. and pharmaceutical preparations by liquid chromatography-fluorescence detection
Determination of hypericin and pseudohypericin in extracts from Hypericum perforatum L. and pharmaceutical preparations by liquid chromatography-fluorescence detection
Zotou, A.; Loukou, Z.
Chromatographia 54(3/4): 218-224
2001
ISSN/ISBN: 0009-5893
DOI: 10.1007/bf02492249
An isocratic reversed-phase liquid chromatographic method for the simultaneous determination of hypericin and pseudohypericin, two of the main constituents of Hypericum perforatum (from northern Greece), has been developed. The compounds were eluted from an Inertsil ODS-3 column by triethylammonium acetate-methanol-acetonirile (5:15:80) eluent and detected fluorimetrically, excitation 478, emission 598 nm.