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Determination of hypericin and pseudohypericin in extracts from Hypericum perforatum L. and pharmaceutical preparations by liquid chromatography-fluorescence detection


Determination of hypericin and pseudohypericin in extracts from Hypericum perforatum L. and pharmaceutical preparations by liquid chromatography-fluorescence detection



Chromatographia 54(3/4): 218-224



ISSN/ISBN: 0009-5893

DOI: 10.1007/bf02492249

An isocratic reversed-phase liquid chromatographic method for the simultaneous determination of hypericin and pseudohypericin, two of the main constituents of Hypericum perforatum (from northern Greece), has been developed. The compounds were eluted from an Inertsil ODS-3 column by triethylammonium acetate-methanol-acetonirile (5:15:80) eluent and detected fluorimetrically, excitation 478, emission 598 nm.

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Accession: 003706338

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