EurekaMag
+ Translate
+ Most Popular
Investigations on the organic drift in North Swedish streams
Limitations of Using Microsoft Excel Version 2016 (MS Excel 2016) for Statistical Analysis for Medical Research
Phloem necrosis of coffee in Surinam
Anatomy of Mystus seenghala IV Nervous system
Integrated farming system for gardenland conditions of Coimbatore district - an over view
Tetrapleure (Tetrapleura tetraptera), an unknown African medicinal and spice plant
The origin and phylogenetic significance of the trochophoran larvae 2. evolutionary significance of the larvae of coelomate worms and mollusks
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
Enterobacter amnigenus. An unusual human pathogen
Influence of Seriboost foliar application on leaf yield and leaf protein content in mulberry (Morus spp.), in relation to silkworm cocoon production
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
'Pan-sukh' disease of Rice in the Central Provinces
Geological age of the Ptilophyllum flora; a critical reassessment
Study of vitellogenesis in birds; physiological phases & role of folliculin in vitellogenesis
Evaluation of WCT coconut and Komadan coconut
Therapy for acne with saccharomyces boulardii
Evidence for Late Cretaceous N-S dextral shear in the west-central crystalline core, North Cascades, Washington
'Rajeshwari' - a high-yielding white seeded variety of sesame for Andhra Pradesh
Manufacture of Ricotta cheese from whey fortified with skim milk powder using different acidulants
Occurrence of Eutrichophilus mexicanus (Rudow, 1866) and Eutrichophilus lobatus (Ewing, 1936) (Phthiraptera: Trichodectidae) on Sphiggurus villosus (Cuvier, 1825) (Rodentia: Erethizontidae) in Rio de
Factors affecting fruitfulness in durian (Durio zibethinus Murr.). I. Flowering and pollination
Bronchial cuff pressure change caused by left-sided double-lumen endobronchial tube displacement
Some Biological Applications of Organometallic Compounds
The composition of pampas-grass (Cortaderia argentea.)

Determination of the attachment of Photobacterium damselae subsp. piscicida to fish cells using an enzyme linked immunosorbent assay


Determination of the attachment of Photobacterium damselae subsp. piscicida to fish cells using an enzyme linked immunosorbent assay



Gyobyo Kenkyu = Fish Pathology 36(4): 201-206



DOI: 10.3147/jsfp.36.201

This paper describes the application of a modified ELISA, which offers a simple and semi-quantitative method to examine the attachment of P. damselae subsp. piscicida to CHSE-214 cells. The suitability of the ELISA procedure to assess this attachment was first confirmed by comparing the results of the ELISA with levels of attachment seen by microscopic examination. Significant differences were observed in the levels of adhesion to CHSE-214 among 32 P. damselae subsp. piscicida isolates from different species of fish and geographically different regions. Exposure of bacteria to low pH (pH 4-6) did not enhance their abilities to adhere to CHSE-214. However, treatment of two representative isolates, Italian I736 and Japanese SP92144, with seven different monosaccharides, revealed differences in their abilities to bind to the cells. Attachment of isolate SP92144 was significantly enhanced by treatment with both N-acetylneuraminic acid and L-fructose, while N-acetyl-D-galactosamine significantly decreased its ability to adhere. The attachment of strain I736 increased significantly when treated with N-acetylneuraminic acid.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003707053

Download citation: RISBibTeXText

Related references

Development of a multiplex PCR assay for Photobacterium damselae subsp. piscicida identification in fish samples. Journal of Fish Diseases 32(8): 645-653, 2009

Identification of iron regulated genes in the fish pathogen Photobacterium damselae subsp piscicida by a Fur Titration Assay. FEMS Congress of European Microbiologists Abstract Book (1): 344, 2003

Immunological and haematological parameters as indicators of fish health status in intensive farming experimental challenge of sea bass with Photobacterium damselae subsp piscida Parametri immunologici ed ematologici quali indicatori dello stato di salute in specie ittiche in allevamento intensivo prove di infezione sperimentale con Photobacterium damselae subsp piscicida in Dicentrarchus labrax. Biologia Marina Mediterranea 132: 34-35, 2006

Development of a real-time PCR assay for rapid detection and quantification of Photobacterium damselae subsp. piscicida in fish tissues. Journal of Fish Diseases 41(2): 247-254, 2018

Existence of two geographically-linked clonal lineages in the bacterial fish pathogen Photobacterium damselae subsp. piscicida evidenced by random amplified polymorphic DNA analysis. Epidemiology and Infection 125(1): 213-219, 2000

Invasion of fish epithelial cells by Photobacterium damselae subsp. piscicida: evidence for receptor specificity, and effect of capsule and serum. Microbiology 146: 21-30, 2000

Binding of haemin by the fish pathogen Photobacterium damselae subsp. piscicida. Diseases of Aquatic Organisms 48(2): 109-115, 2002

Photobacterium damselae subsp. piscicida: an integrated view of a bacterial fish pathogen. International Microbiology: the Official Journal of the Spanish Society for Microbiology 5(1): 3-9, 2002

Invasion and replication of Photobacterium damselae subsp. piscicida in fish cell lines. Journal of Aquatic Animal Health 15(2): 167-174, 2003

Fish photobacteriosis-The importance of rapid and accurate identification of Photobacterium damselae subsp. piscicida. Journal of Fish Diseases 42(8): 1201-1209, 2019

Genetic characterization of pPHDP60, a novel conjugative plasmid from the marine fish pathogen Photobacterium damselae subsp. piscicida. Plasmid 70(1): 154-159, 2013

Characterization of heme-uptake operons in the fish pathogens Photobacterium damselae subsp piscicida and Vibrio anguillarum. FEMS Congress of European Microbiologists Abstract Book (1): 338, 2003

Antimicrobial substances from rhizomes of the giant knotweed Polygonum sachalinense against the fish pathogen Photobacterium damselae subsp. piscicida. Zeitschrift für Naturforschung. C Journal of Biosciences 60(1-2): 39-44, 2005

Use of random amplification to develop a PCR detection method for the causative agent of fish pasteurellosis, Photobacterium damselae subsp. piscicida (Vibrionaceae). Aquaculture 207(3/4): 187-202, 2002

Subtractive hybridization reveals a high genetic diversity in the fish pathogen Photobacterium damselae subsp. piscicida: evidence of a SXT-like element. Microbiology 151(Pt 8): 2659-2669, 2005