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Development of EST-PCR markers for DNA fingerprinting and genetic relationship studies in blueberry (Vaccinium, section Cyanococcus)


Development of EST-PCR markers for DNA fingerprinting and genetic relationship studies in blueberry (Vaccinium, section Cyanococcus)



Journal of the American Society for Horticultural Science 128(5): 682-690



ISSN/ISBN: 0003-1062

Because randomly amplified polymorphic DNA (RAPD) is the only type of molecular marker that has been used extensively in blueberry (Vaccinium spp.) for mapping and DNA fingerprinting of cultivars, there is a need to develop a new, robust marker system. Expressed sequence tags (ESTs) produced from a cDNA library, derived from RNA from floral buds of cold acclimatized plants, were used to develop EST-PCR markers for blueberry. Thirty clones were single-pass sequenced from the 5' and 3' ends.

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Accession: 003708474

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