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Development of a conditional lethal system for a Streptomyces lividans strain and its use to investigate conjugative transfer in soil

Development of a conditional lethal system for a Streptomyces lividans strain and its use to investigate conjugative transfer in soil

FEMS microbiology ecology 38(2-3): 115-121

ISSN/ISBN: 0168-6496

DOI: 10.1016/s0168-6496(01)00183-0

In Streptomyces lividans, a conditional lethal mechanism was obtained by inactivation of gylB in the glycerol operon. The resulting strain was efficiently counter-selected on glycerol-containing medium but the mutation had no effect on the capacity of the strain to be maintained in non-sterile soil samples and to transfer derivatives of the genetic element pSAM2. However, despite the efficiency of the donor counter-selection system, the transfer of a pSAM2 derivative from S. lividans to indigenous soil bacteria was not detected.

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Accession: 003708670

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