Development of a method for agar culture of Monochoria vaginalis using the water extract of unhulled rice
Development of a method for agar culture of Monochoria vaginalis using the water extract of unhulled rice
Koarai, A.; Morita, H.; Hattori, M.; Shibayama, H.
Journal of Weed Science and Technology 47(1): 14-19
2002
One hundred grams of unhulled rice or 20 grams of rice hull were extracted with 1000 ml water at 5 degrees C during 48 hours. Thereafter, the water was filtered and diluted two-fold with distilled water and was used for 0.5% agar culture media in plant boxes, on which seeds of M. vaginalis were sown at a 1-mm depth. Distilled water was used for the 0.5% agar culture media in the boxes as untreated control.