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Development of a new method for measuring the rates of RuBP carboxylase degradation


Development of a new method for measuring the rates of RuBP carboxylase degradation



Agronomia Lusitana 47: 351-357



A new method for measuring the rates of ribulose-bisphosphate (RuBP) carboxylase degradation was developed. The new method involves efficient purification of the enzyme from a desalted extract of duckweed (Lemna minor), the production of anti-RuBP carboxylase antibodies, double labelling of the leaf proteins with 3H and 35S methionine, and the immunoprecipitation of RuBP carboxylase.

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Related references

The response to ribulose bisphosphate(4-) (RuBP (4-)) and RuBP-Mg (2-) in catalysis by structurally divergent RuBP carboxylase/oxygenases. Photosynthesis Research 4(2): 111-117, 1983

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