Development of a polymerase chain reaction and restriction typing assay for the diagnosis of bovine herpesvirus 1, bovine herpesvirus 2, and bovine herpesvirus 4 infections

De-Giuli, L.; Magnino, S.; Vigo, P.Giorgio.; Labalestra, I.; Fabbi, M.

Journal of Veterinary Diagnostic Investigation Official Publication of the American Association of Veterinary Laboratory Diagnosticians Inc 14(4): 353-356

2002


ISSN/ISBN: 1040-6387
PMID: 12152821
DOI: 10.1177/104063870201400417
Accession: 003708939

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Abstract
A multiplex polymerase chain reaction (PCR) method coupled with a restriction analysis of PCR products (PCR with restriction fragment length polymorphism) was developed for the simultaneous detection of bovine herpesvirus 1, bovine herpesvirus 2 and bovine herpesvirus 4 infections. The specificity, sensitivity and practical diagnostic applicability of this method were evaluated. This assay may be also adapted to the diagnosis of suid herpesvirus 1 and equine herpesviruses 1 and 3 and could become a powerful diagnostic tool.