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Development of an enzyme immunoassay for the determination of testosterone


Development of an enzyme immunoassay for the determination of testosterone



Turk Veterinerlik ve Hayvanclk Dergisi 27(1): 45-50



In this study, a double antibody enzyme immunoassay for the direct determination of testosterone in plasma was developed. Testosterone 3-O-CMO was conjugated with horseradish peroxidase by the mixed anhydride method, and the conjugate purified by column chromatography (sephadex G-25). Testosterone antibody was obtained by immunization of rabbits against Testosterone-3-O-CMO-BSA. Cross reactions of the antiserum against some steroids were found to be <0.01%.

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