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Development of mouse embryos derived from oocytes reconstructed by metaphase I spindle transfer

Development of mouse embryos derived from oocytes reconstructed by metaphase I spindle transfer

Zygote 11(1): 53-59

ISSN/ISBN: 0967-1994

PMID: 12625529

DOI: 10.1017/s0967199403001072

Abnormal oocyte spindle is frequently associated with the infertility of aged women. Directly manipulating the metaphase I (MI) spindle may be a feasible method to overcome this kind of problem. Here, we report that the MI meiotic spindle can be removed from MI mouse oocytes and will autonomously divide into two daughter cells with the same size, morphology and an equal number of chromosomes after culture for 5 h in maturation medium.

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Accession: 003709758

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