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Development of multiplexed fluorescence microbead covalent assays (FMCAs) for pesticide biomonitoring

Development of multiplexed fluorescence microbead covalent assays (FMCAs) for pesticide biomonitoring

Bulletin of Environmental Contamination and Toxicology 68(4): 470-477

ISSN/ISBN: 0007-4861

PMID: 12069049

DOI: 10.1007/s001280278

This paper describes the use of multiplexed fluorescence microbead covalent assays (FMCAs) for atrazine (ATR) and metolachlor mercapturate (MM), both in the monoplexed and multiplexed format. ATR and its metabolites have been measured by HPLC coupled with HPLC-MS/MS using atmospheric pressure chemical ionization. The multiplexed FMCAs presented were observed to have substantial benefits over the classical chemical and traditional ELISA methods. The ability to multiplex, the savings in time, minimization of systematic error, the "no wash" format and improved reaction kinetics with sensitivity and precision comparable to ELISA methods suggest that multiplexed FMCA for pesticides/pesticide metabolites is a viable adjunct to classical instrumental methods and ELISA methods for urinary biological monitoring.

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Accession: 003709767

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