EurekaMag
+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Developmental potential of bovine nuclear transfer embryos and postnatal survival rate of cloned calves produced by two different timings of fusion and activation


Developmental potential of bovine nuclear transfer embryos and postnatal survival rate of cloned calves produced by two different timings of fusion and activation



Molecular Reproduction and Development 66(3): 264-272



ISSN/ISBN: 1040-452X

PMID: 14502605

DOI: 10.1002/mrd.10352

We compared developmental potential of somatic cell nuclear transfer (NT) embryos and postnatal survivability of cloned calves produced by two different fusion and activation protocols. As donor cells for NT, bovine cumulus cell-derived cultured cells of passage 5 were used following culture in serum-starved medium for 5-7 days. Enucleated oocytes were fused with donor cells at 21 or 24 hr post maturation. NT embryos fused at 21 hr were activated chemically 3 hr after fusion (DA group) and embryos fused at 24 hr were activated chemically immediately after fusion (FA group). Chemical activation was accomplished by calcium ionophore for 5 min and cytochalasin D + cycloheximide for 1 hr then cycloheximide alone for 4 hr. After in vitro culture in IVD101 medium for 7 days, embryo transfer was performed. Fusion rates were 86 and 84% in the DA and FA groups, respectively. Developmental rate to the blastocyst stage of NT embryos in the DA group was higher than in the FA group (42% vs. 28%). Pregnancy rate did not differ significantly between the DA and FA groups (11/13 and 5/7 at day 35), and 13 cloned calves (including 1 set of twins from a single embryo transfer) were born. High rates of postnatal mortality were observed in both groups. These results suggest that the DA method improves in vitro developmental potential of NT embryos, but the timing of fusion and chemical activation does not affect the pregnancy rate and the survivability of cloned calves.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003710564

Download citation: RISBibTeXText

Related references

Nuclear Modeling and Developmental Potential of Bovine Somatic Nuclear Transfer Embryos Cloned by Two Different Activation Methods. Reproductive and Developmental biology 35(1): 105-113, 2011

Developmental potential of rabbit nuclear transfer embryos produced by various fusion/activation protocols. Theriogenology 53(1): 230, 2000

The timing of fusion and chemical activation in nuclear transfer affects developmental potential of bovine embryos. Theriogenology 55(1): 252, 2001

In Vitro Developmental Potential of Nuclear Transfer Embryos Cloned with Enucleation Methods using Pre-denuded Bovine Oocytes. Reproduction in Domestic Animals (1990) 46(6): 1035-1042, 2011

In vitro developmental potential of nuclear transfer embryos cloned with enucleation methods using pre-denuded bovine oocytes. Reproduction in Domestic Animals 46(6): 1035-1042, 2011

Relationship between activation and fusion on developmental ability of bovine nuclear transfer embryos. Theriogenology 55(1): 239, 2001

Developmental rate and ploidy of embryos produced by nuclear transfer with different activation treatments in cattle. Animal Reproduction Science 92(1-2): 37-49, 2005

Developmental potential of bovine nuclear transfer embryos produced using different types of adult donor cells. Theriogenology 53(1): 210, 2000

Development of bovine cloned embryos produced by nuclear transfer of embryonic cultured cells isolated from somatic cell nuclear transfer blastocysts. Reproduction Fertility and Development 20(1): 92, 2008

Development of bovine cloned embryos produced by nuclear transfer of embryonic cultured cells isolated from somatic cell nuclear transfer blastocysts. Reproduction Fertility and Development 20(1): 92-92, 2008

Effect of timing of oocyte-cell fusion on developmental potential of bovine nuclear transfer embryos from cumulus cells. Theriogenology 59(1): 232, 2003

Pregnancies produced by bovine embryos cloned by nuclear transfer. Archivos de Medicina Veterinaria 39(1): 59-62, 2007

Effect of linoleic acid-albumin on the developmental potential of embryos produced by nuclear transfer into frozen-thawed bovine cytoplasts. Theriogenology 53(1): 256, 2000

Comparison of gene transcription in cloned bovine embryos produced by different nuclear transfer techniques. Molecular Reproduction and Development 60(3): 281-288, 2001

Production of Bovine Embryos and Calves Cloned by Nuclear Transfer Using Mesenchymal Stem Cells from Amniotic Fluid and Adipose Tissue. Cellular Reprogramming 18(2): 127-136, 2016