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Developmentally-regulated changes of Xist RNA levels in single preimplantation mouse embryos, as revealed by quantitative real-time PCR

Developmentally-regulated changes of Xist RNA levels in single preimplantation mouse embryos, as revealed by quantitative real-time PCR

Molecular Reproduction and Development 61(4): 425-436

ISSN/ISBN: 1040-452X

PMID: 11891913

DOI: 10.1002/mrd.10037

Xist RNA localizes to the inactive X chromosome in cells of late cleavage stage female mouse embryos. Fluorescence in situ hybridization (FISH), however, does not quantify the number of Xist transcripts per nucleus. In the present study, real-time reverse transcription-polymerase chain reaction (RT-PCR) was used to measure Xist RNA levels in single preimplantation embryos and to establish developmental profiles in both female and male samples.

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Accession: 003710649

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