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Differential expression of G6PDH mRNA transcripts during Kunming mouse preimplantation embryo development in CZB and KSOM


Differential expression of G6PDH mRNA transcripts during Kunming mouse preimplantation embryo development in CZB and KSOM



Chinese Journal of Veterinary Science 22(4): 359-362



Inner pair primers and outer pair primers were designed and synthesized according to the cDNA sequence of glucose-6-phosphate dehydrogenase (G6PDH) in mouse muscles. Nested reverse transcriptase-polymerase chain reaction (nested RT-PCR) was used to determine the pattern of expression of mRNA encoding G6PDH throughout Kunming mouse 2- and 4-cell embryo culture in vitro in CZB and KSOM.

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