+ Translate
+ Most Popular
Investigations on the organic drift in North Swedish streams
Limitations of Using Microsoft Excel Version 2016 (MS Excel 2016) for Statistical Analysis for Medical Research
Phloem necrosis of coffee in Surinam
Anatomy of Mystus seenghala IV Nervous system
Integrated farming system for gardenland conditions of Coimbatore district - an over view
Tetrapleure (Tetrapleura tetraptera), an unknown African medicinal and spice plant
The origin and phylogenetic significance of the trochophoran larvae 2. evolutionary significance of the larvae of coelomate worms and mollusks
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
Enterobacter amnigenus. An unusual human pathogen
Influence of Seriboost foliar application on leaf yield and leaf protein content in mulberry (Morus spp.), in relation to silkworm cocoon production
The identity of the lipstick mold of cultivated mushrooms agaricus bisporus
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
'Pan-sukh' disease of Rice in the Central Provinces
Geological age of the Ptilophyllum flora; a critical reassessment
Study of vitellogenesis in birds; physiological phases & role of folliculin in vitellogenesis
Evaluation of WCT coconut and Komadan coconut
Therapy for acne with saccharomyces boulardii
Evidence for Late Cretaceous N-S dextral shear in the west-central crystalline core, North Cascades, Washington
'Rajeshwari' - a high-yielding white seeded variety of sesame for Andhra Pradesh
Manufacture of Ricotta cheese from whey fortified with skim milk powder using different acidulants
Occurrence of Eutrichophilus mexicanus (Rudow, 1866) and Eutrichophilus lobatus (Ewing, 1936) (Phthiraptera: Trichodectidae) on Sphiggurus villosus (Cuvier, 1825) (Rodentia: Erethizontidae) in Rio de
Factors affecting fruitfulness in durian (Durio zibethinus Murr.). I. Flowering and pollination
Bronchial cuff pressure change caused by left-sided double-lumen endobronchial tube displacement
Some Biological Applications of Organometallic Compounds
The composition of pampas-grass (Cortaderia argentea.)

Differential gene expression in pre-implantation embryos from mouse oocytes injected with round spermatids or spermatozoa

Differential gene expression in pre-implantation embryos from mouse oocytes injected with round spermatids or spermatozoa

Human Reproduction 16(7): 1449-1456

ISSN/ISBN: 0268-1161

PMID: 11425828

DOI: 10.1093/humrep/16.7.1449

BACKGROUND: The use of immature male germ cells to fertilize human oocytes raises several questions. Spermatozoa are normally quiescent, but many genes are transcribed post-meiotically in round spermatids. This creates a novel situation for the oocyte. We have therefore explored the effects on early embryonic development of introducing a fully transcriptionally active round spermatid into the oocyte. METHODS AND RESULTS: Following the micro-injection of spermatozoa or spermatids into mouse oocytes we have analysed the expression, at various times, of six genes in the resulting embryo. Spermatozoa and spermatids produced similar fertilization rates. Hprt was expressed in all embryos at all stages tested. Hsp70.1 was found normally during the 2-cell stage and repressed by the 4-cell stage in embryos from both spermatozoa and round spermatids. However, the amplitude of the signal was greatly reduced in 2-cell embryos from round spermatids. Smcy also showed a disturbed pattern of expression in embryos from round spermatids. Protamine 2, which is normally restricted to the spermatid stage, was expressed following fertilization with round spermatids, but was already repressed at the two pronuclei stage. Ube1Y, which is normally expressed post-meiotically and not during the post-implantatory development, was expressed up to the 2-cell stage in embryos from round spermatids only, and then repressed. Ube1X was also expressed up to the 2-cell stage, but in both embryo types. CONCLUSIONS: We therefore suspect that in embryos fertilized with round spermatids, regulatory mechanisms for inhibiting the inappropriate transcription of male post-meiotically expressed genes are activated following fertilization, permitting the zygotic genome activation to occur, though with some disturbances.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003713575

Download citation: RISBibTeXText

Related references

Mouse preimplantation embryos developed from oocytes injected with round spermatids or spermatozoa have similar but distinct patterns of early messenger RNA expression. Biology of Reproduction 69(4): 1170-1176, 2003

Development of normal offspring from mouse oocytes injected with mature spermatozoa, testicular spermatozoa, spermatids and secondary spermatocytes. Biology of Reproduction 52(Suppl. 1): 139, 1995

Mouse oocytes injected with testicular spermatozoa or round spermatids can develop into normal offspring. Development 121(8): 2397-2405, 1995

Mouse oocytes injected with cryopreserved round spermatids can develop into normal offspring. Journal of Assisted Reproduction and Genetics 13(5): 431-434, 1996

Expression and localization of Npr2 in mouse oocytes and pre-implantation embryos. Biotechnic and Histochemistry: Official Publication of the Biological Stain Commission 94(5): 320-324, 2019

Development of mouse embryos after fusion of oocytes with testicular round spermatids. Theriogenology 41(1): 232, 1994

Embryonic death at implantation after induction of dominant lethal mutations in spermatids and spermatozoa of the mouse. Journal of Reproduction and Fertility 46(2): 485-487, 1976

Adenophostin, a potent agonist of the inositol 1,4,5-trisphosphate receptor, is useful for fertilization of mouse oocytes injected with round spermatids leading to normal offspring. Biology of Reproduction 58(3): 867-873, 1998

The response of mouse oocytes injected with sea urchin spermatozoa. Zygote 5(3): 229-234, 1997

Promoter-specific expression of the imprinted IGF2 gene in bovine oocytes and preimplantation embryos. Reproduction in Domestic Animals 2021, 2021

Partial reprogramming of imprinted gene expression during post-implantation development of mouse embryos exposed to TSA but not 5AzaC during pre-implantation. Reproduction in Domestic Animals 47: 506-507, 2012

Inhibition of zona pellucida gene expression by antisense oligonucleotides injected into mouse oocytes. Journal of Biological Chemistry 270(2): 849-853, 1995

Differential regulation of G protein subunit expression in mouse oocytes, eggs, and early embryos. Developmental Biology 142(1): 129-137, 1990

Expression of the rig gene in mouse oocytes and early embryos. Molecular Reproduction and Development 28(4): 319-324, 1991

Fertilization and development of mouse oocytes injected with membrane-damaged spermatozoa. Human Reproduction 12(12): 2797-2801, 1997