+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Differential secretion of chicken growth hormone variants after growth hormone-releasing hormone stimulation in vitro

Differential secretion of chicken growth hormone variants after growth hormone-releasing hormone stimulation in vitro

Endocrine 17(2): 91-102

ISSN/ISBN: 1355-008X

PMID: 12041920

DOI: 10.1385/endo:17:2:91

Variants of growth hormone (GH) are present in most vertebrates. Chicken GH (cGH) undergoes posttranslational modifications that contribute to its structural diversity. Although the 22-kDa form of GH is the most abundant, some other variants have discrete bioactivities that may not be shared by others. The proportion of cGH variants changes during ontogeny, suggesting that they are regulated differentially. The effect of growth hormone-releasing hormone (GHRH) on the release of cGH variants was studied in both pituitary gland and primary cell cultures, employing sodium dodecyl sulfate polyacrylamide gel electrophoresis, Western blotting, and densitometry. GHRH (2 nM, 2 h) stimulated the secretion of most of the size variants of cGH although the amplitude of increase was not equal for each one. A differential effect on the secretion of GH size variants, particularly on the 22- (monomer) and 26-kDa (putatively glycosylated) cGH isoforms was found in both systems. In the whole pituitary culture, the proportion of the 26-kDa immunoreactive cGH increased 35% while the 22 kDa decreased 31% after GHRH treatment in comparison with the controls. In the primary cell culture system, the proportion of the glycosylated variant increased 43% whereas the monomer and the dimer decreased 22.26 and 29%, respectively, after GHRH stimulation. Activators of intracellular signals such as 1 mM 8-bromo-cAMP and 1 microM phorbol myristate acetate had a similar effect to that obtained with GHRH. The data support the hypothesis that GH variants may be under differential control and that GHRH promotes the release of a glycosylated cGH variant that has an extended half-life in circulation.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 003713821

Download citation: RISBibTeXText

Related references

Growth hormone secretion from chicken adenohypophyseal cells in primary culture: effects of human pancreatic growth hormone-releasing factor, thyrotropin-releasing hormone, and somatostatin on growth hormone release. General and Comparative Endocrinology 65(3): 408-414, 1987

Combined growth hormone-releasing hormone and growth hormone-releasing peptide-6 test for the evaluation of growth hormone secretion in children with growth hormone deficiency and growth hormone neurosecretory dysfunction. Hormone Research 70(4): 215-223, 2008

Growth hormone/insulin-like growth factor-1 response to acute and chronic growth hormone-releasing peptide-2, growth hormone-releasing hormone 1-44NH2 and in combination in older men and women with decreased growth hormone secretion. Endocrine 14(1): 79-86, 2001

Potent interaction between trh and human pancreatic growth hormone releasing factor in stimulating chicken growth hormone in vivo hypothalamic noradrenergic mediation in trh stimulation of chicken growth hormone release. Domestic Animal Endocrinology 2(4): 183-190, 1985

Growth hormone releasing hormone and growth hormone releasing peptide-6 act synergistically to stimulate growth hormone secretion in hypopituitary patients with growth hormone deficiency. Journal of Endocrinology 152(Suppl. ): P29, 1997

Spontaneous daily fluctuations and the effect of thyrotropin releasing hormone melanocyte stimulating hormone release inhibiting hormone and luteinizing hormone releasing hormone on growth hormone secretion during florid acromegaly. Verhandlungen der Deutschen Gesellschaft fuer Innere Medizin 81: 1521-1523, 1975

Growth hormone secretion in anaesthetized fowl. 1. Refractoriness to repeated stimulation by human pancreatic growth hormone-releasing factor (hpGRF) or thyrotrophin releasing hormone (TRH). General and Comparative Endocrinology 59(1): 1-9, 1985

Growth hormone releasing hormone-sensitive adenylate cyclase activity in growth hormone-producing pituitary adenoma: correlation to the response of plasma growth hormone to growth hormone releasing hormone in patients with acromegaly. Endocrinologia Japonica 36(2): 229-236, 1989

Growth hormone therapy in normal short children induces a transitory decrease in plasma growth hormone releasing hormone levels and in human growth hormone responsiveness to exogenous growth hormone releasing hormone. Journal of Endocrinological Investigation 15(10): 735-739, 1992

Comparative stimulation of growth hormone secretion in anaesthetized chickens by human pancreatic growth hormone-releasing factor (hpGRF) and thyrotrophin-releasing hormone (TRH). Neuroendocrinology 39(4): 314-320, 1984

Pulsatile growth hormone releasing hormone treatment can induce growth hormone and somatomedin c responses in growth hormone deficient children initially unresponsive to single bolus growth hormone releasing hormone. Pediatric Research 20(4 Part 2): 217A, 1986

Stimulation of growth hormone secretion in dwarf chickens by thyrotrophin-releasing hormone (TRH) or human pancreatic growth-hormone-releasing factor (hpGRF). General and Comparative Endocrinology 55(3): 493-497, 1984

Insulin suppresses basal growth hormone secretion and inhibits stimulation of growth hormone by growth hormone releasing factor in rat pituitary cells. Clinical Research 33(1): 95A, 1985

Atropine selectively blocks growth hormone releasing hormone induced growth hormone secretion without altering luteinizing hormone fsh tsh prolactin and acth cortisol secretion elicited by their specific hypothalamic releasing factors. Clinical Endocrinology 25(3): 319-324, 1986

Growth hormone secretion during sleep and growth hormone releasing factor stimulation in growth hormone deficient and short normal children. Pediatric Research 20(11): 1189, 1986