Direct induction of T lymphocyte-specific gene expression by the mammalian Notch signaling pathway
Direct induction of T lymphocyte-specific gene expression by the mammalian Notch signaling pathway
Reizis, B.; Leder, P.
Genes and Development 16(3): 295-300
2002
ISSN/ISBN: 0890-9369
PMID: 11825871
DOI: 10.1101/gad.960702
The Notch signaling pathway regulates the commitment and early development of T lymphocytes. We studied Notch-mediated induction of the pre-T cell receptor alpha (pTa) gene, a T-cell-specific transcriptional target of Notch. The pTa enhancer was activated by Notch signaling and contained binding sites for its nuclear effector, CSL. Mutation of the CSL-binding sites abolished enhancer induction by Notch and delayed the up-regulation of pTa transgene expression during T cell lineage commitment. These results show a direct mechanism of stage- and tissue-specific gene induction by the mammalian Notch/CSL signaling pathway.