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Direct regeneration of transgenic buckwheat from hypocotyl segment by Agrobacterium-mediated transformation


Direct regeneration of transgenic buckwheat from hypocotyl segment by Agrobacterium-mediated transformation



Korean Journal of Crop Science 46(5): 375-379



Transgenic plants from hypocotyl segments of buckwheat were produced with the Agrobacterium tumefaciens strain LBA4404 harbouring the binary vector pBI121 containing chimaeric genes of neomycin phosphotransferase II (npt II) and beta -glucuronidase (gus). Two weeks after co-cultivation with A. tumefaciens, most of the hypocotyl segments gradually became brown and died on the selection medium containing 100 mg/litre of kanamycin.

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