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Disarming of wild type Agrobacterium rhyzogenes K599

Disarming of wild type Agrobacterium rhyzogenes K599

Hereditas Beijing 23(4): 336-340

The new plasmid pXT3sacB was constructed from pJBJ106, pBluescript SK(+) and pUCD800. The wild-type T-DNA of Agrobacterium rhizogenes K599, which induces hair roots, was deleted using pXT3sac by homologous recombination. A disarming K599 contains selective markers for resistance to ampicillin/carbenicillin, and the growth inhibition by 10% sucrose.

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