EurekaMag
+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Down-regulation of DR12, an auxin-response-factor homolog, in the tomato results in a pleiotropic phenotype including dark green and blotchy ripening fruit


Down-regulation of DR12, an auxin-response-factor homolog, in the tomato results in a pleiotropic phenotype including dark green and blotchy ripening fruit



Plant journal: for cell and molecular biology 32(4): 603-613



ISSN/ISBN: 0960-7412

PMID: 12445130

DOI: 10.1046/j.1365-313x.2002.01450.x

Following differential screening of gene expression during tomato fruit development, we isolated developmentally regulated (DR) clones, including several putative transcription factors. Based on sequence homology, DR1, DR3, DR4 and DR8 are members of the Aux/IAA family, and DR12 belongs to the auxin response factor (ARF) family of transcription factors. Importantly, mRNA accumulation for the Aux/IAA-like genes was regulated by ethylene in tomato fruit but not in the leaves, indicating that these putative auxin response components also participate to the ethylene-dependent regulation of gene expression in a tissue-specific manner. The functional significance of DR12, the ARF-like gene, was investigated by cellular biology and reverse genetics approaches. Heterologous protein targeting studies, carried out using a DR12-GFP gene fusion construct, revealed specific nuclear localization of the DR12-encoded protein, in accordance with its putative function as a transcriptional regulator. Transgenic plants over- and under-expressing DR12 were generated in order to explore the physiological role of the gene. Both antisense and sense co-suppressed DR12-inhibited lines displayed a pleiotropic phenotype that included dark-green immature fruit, unusual cell division in the fruit pericarp, blotchy ripening, enhanced fruit firmness, upward curling leaves and increased hypocotyl and cotyledon growth. While a perturbation of the response to auxin may explain some of the phenotypes, surprisingly, the expression of members of four classes of early auxin-regulated genes was unaffected in the DR12-inhibited plants. The involvement of this ARF-like encoded protein in mediating the auxin response is discussed along with the possibility that it might affect responsiveness to other phytohormones in the tomato.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003718731

Download citation: RISBibTeXText

Related references

AUXIN RESPONSE FACTOR 2 Intersects Hormonal Signals in the Regulation of Tomato Fruit Ripening. Plos Genetics 12(3): E1005903, 2016

Auxin Response Factor SlARF2 Is an Essential Component of the Regulatory Mechanism Controlling Fruit Ripening in Tomato. Plos Genetics 11(12): E1005649, 2015

Auxin drives tomato spotted wilt virus (TSWV) resistance through epigenetic regulation of auxin response factor ARF8 expression in tomato. Gene 2021: 145905, 2021

Auxin Response Factor 2A Is Part of the Regulatory Network Mediating Fruit Ripening Through Auxin-Ethylene Crosstalk in Durian. Frontiers in Plant Science 11: 543747, 2020

Effect of soil water on brown blotchy ripening in tomato fruit. China Vegetables 3: 8-10, 2000

Protein and enzyme changes in tomato fruit in relation to blotchy ripening and potassium nutrition. Journal of the Science of Food and Agriculture 27(1): 15-22, 1976

Blotchy ripening of tomato fruit: a preliminary report on some chemical aspects of the disorder. A.R. Cheshunt exp. Res. Stat. 1956. 50-4, 1956

Tomato AUXIN RESPONSE FACTOR 5 regulates fruit set and development via the mediation of auxin and gibberellin signaling. Scientific Reports 8(1): 2971, 2018

Multicompartmental analysis on the metabolism of 14C glucose in tomato fruit in the inducing condition of blotchy ripening. Nippon dojohiryogaku zasshi Journal of the science of soil and manure Japan: 5, 48 (9 10) 446-448, 1977

The Solanum lycopersicum auxin response factor 7 (SlARF7) regulates auxin signaling during tomato fruit set and development. Plant Journal: for Cell and Molecular Biology 57(1): 160-170, 2009

The effect of heavy dressings of phosphate and lime on the incidence of cloud blotchy ripening) in tomato fruit. Trien. Rep. Caw thron Inst. 1966. 28-31, 1966

The effect of heavy dressings of phosphorus and lime on the incidence of cloud (blotchy-ripening) in tomato fruit. A.R. Cawthron Inst. 61, 1961. 38-40, 1961

The effect of heavy dressings of phosphate and lime on the incidence of cloud (blotchy-ripening) in tomato fruit. Bienn. Rep. Cawthron Inst, 34-6, 1963

Transcriptional regulation ofPaPyls,PaPp2CsandPaSnRk2sduring sweet cherry fruit development and in response to abscisic acid and auxin at onset of fruit ripening. Plant Growth Regulation 75(2): 455-464, 2015

The Solanum lycopersicum AUXIN RESPONSE FACTOR 7 (SlARF7) mediates cross-talk between auxin and gibberellin signalling during tomato fruit set and development. Journal of Experimental Botany 62(2): 617-626, 2011