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Effect of donor strain and maturation stage of rabbit oocytes on results of penetration test of rabbit semen



Effect of donor strain and maturation stage of rabbit oocytes on results of penetration test of rabbit semen



World Rabbit Science 10(2): 53-62



The objective of this work was to develop a homologous in vitro penetration test to evaluate the behaviour of rabbit spermatozoa. Three treatments were applied to spermatozoa (fresh, treated with heparin and frozen with a DMSO-sucrose extender), and two types of oocytes (immature or in vitro matured) belonging to four different rabbit lines were used. The test was performed in TCM-199, under the following conditions: 37 degrees C, 5% CO2 and saturated humidity for 6 h.

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Accession: 003727453

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