+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Effect of dyskinetoplastic agents on ultrastructure and oxidative phosphorylation in Crithidia fasciculata



Effect of dyskinetoplastic agents on ultrastructure and oxidative phosphorylation in Crithidia fasciculata



Biocell 25(1): 43-51



Ethidium bromide (EB) is an intercalating agent which binds specifically to the kinetoplast (mitochondrial) DNA (kDNA) of trypanosomatids. Accordingly, EB inhibits DNA replication, thus inducing dyskinetoplasty. Since in eukariotic organisms mitochondrial DNA encodes the genetic information for cytochromes b, aa3 and F0F1 ATPase, it seemed of interest to establish whether a similar effect occurs in Crithidia fasciculata, a trypanosomatid used for assay of potential trypanocidal drugs. Culturing of C. fasciculata in the presence of EB inhibited growth and induced dyskinetoplasty, as confirmed by electron microscopy. The kinetoplast of EB-cultured crithidia lost its characteristic arc shape, it was misplaced in the cell cytoplasm its matrix structure and membrane differentiation were specifically modified. Dyskinetoplasty decreased crithidia respiration and oxidative phosphorylation, as indicated by the lower ATP level, ATP/ADP ratio and adenylate energy charge. The interference of EB with kinetoplastic constituents synthesis was confirmed by the lack of action of EB on crithidia in the stationary phase of growth, that ruled out direct inhibition of oxidative phosphorylation enzymes. The lipophilic o-naphthoquinone [beta]-lapachone produced structural alterations in kinetoplast membranes, that correlated with inhibition of oxidative phosphorylation. These latter effects involved free radicals since they were prevented by free radical scavengers.

(PDF emailed within 1 workday: $29.90)

Accession: 003727600

Download citation: RISBibTeXText

PMID: 11387876


Related references

Oxidative phosphorylation in Crithidia fasciculata. Dissertation Abstr lnt, 34B9: 4212, 1974

Oxidative phosphorylation in crithidia fasciculata. Biochemical and Biophysical Research Communications 28(5): 821-826, 1967

Fate of kinetoplast DNA in dyskinetoplastic strains of Trypanosoma equiperdum and Crithidia fasciculata. Journal of Protozoology 23(2): 17A, 1976

Inhibition by suramin of oxidative phosphorylation in Crithidia fasciculata. Comparative biochemistry and physiology B Comparative biochemistry1(4): 611-616, 1982

Metabolism of folate and related compounds in a trypanosomid flagellate, Crithidia fasciculata, Part IV. Promoting effect of methotrexate and aminopterin on the growth of Crithidia fasciculata. Agricultural and Biological Chemistry 47(7): 1651-1652, 1983

Physiological adaptation of Crithidia fasciculata to carbonyl cyanide m-chlorophenylhydrazone, an uncoupler of oxidative phosphorylation. Abstracts of papers read at the 5th International Congress on Protozoology, New York City, 26 June 2 July, 1977: 113, 1977

Inhibition of oxidative phosphorylation in Crithidia fasciculata and Trypanosoma cruzi by lipophilic o-quinones and nifurtimox. Revista Argentina de Microbiologia 26(2): 72-86, 1994

Physiological adaptation of Crithidia fasciculata to carbony cyanide m-chlorophenylhydrazone, an uncoupler of oxidative phosphorylation. Dissertation Abstracts International 39B(2): 598, 1978

Induced resistance of crithidia fasciculata kinetoplastida trypanosomatidae to carbonyl cyanide m chlorophenyl hydrazone an uncoupler of oxidative phosphorylation. ASB Bulletin 24(2): 64, 1977

Metabolism of folate and related compounds in a trypanosomid flagellate, Crithidia fasciculata. Part III. Effect of configuration of neopterin isomers on the activity of pteridine reductase: Dihydrofolate reductase from Crithidia fasciculata. Agricultural and Biological Chemistry 47(2): 425-427, 1983

Comparative analysis of respiratory chain and oxidative phosphorylation in Leishmania tarentolae, Crithidia fasciculata, Phytomonas serpens and procyclic stage of Trypanosoma brucei. Molecular and Biochemical Parasitology 193(1): 55-65, 2014

The effect of short wavelength uv light on antigens lectin receptors and the ultrastructure of crithidia fasciculata. Brazilian Journal of Medical & Biological Research 22(3): 345-350, 1989

The effect of short-wavelength ultraviolet light on antigens, lectin receptors and the ultrastructure of Crithidia fasciculata. Brazilian Journal of Medical and Biological Research 22(3): 345-350, 1989

Effect of ethidium bromide on the oxidative metabolism and enzyme profiles of Crithidia fasciculata. Journal of Protozoology 24(1): 192-195, 1977

Metabolism of folate and related compounds in a trypanosomid flagellate, Crithidia fasciculata. Part VI. Purification and some properties of folate-hydrolyzing enzyme from Crithidia fasciculata. Agricultural and Biological Chemistry 48(2): 505-511, 1984