EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Effect of ethylene and 1-methylcyclopropene on chlorophyll catabolism of broccoli florets



Effect of ethylene and 1-methylcyclopropene on chlorophyll catabolism of broccoli florets



Plant growth regulation 40(1): 33-38



Branchlets of broccoli (Brassica oleracea L.) were used to examine ethylene-stimulated chlorophyll catabolism. Branchlets treated with: 1) air (CK); 2) 1 microliters (.) L-1 1-methylcyclopropene (1-MCP) for 14 hr at 20 degrees C; 3) 1000 microliters (.) L-1 ethylene (C2H4) for 5 hr at 20 degrees C; or 4) 1-MCP then C2H4, were stored in the dark at 20 degrees C for up to 3 d. Chlorophyll (Chl) content and branchlet hue angle decreased during the storage period and 1-MCP treatment delayed this change. Chl degradation in broccoli was accelerated by exposure to C2H4, especially for Chl a. Prior treatment with 1-MCP prevented degreening stimulated by C2H4. Lipoxygenase activity was not altered by any of the treatments, however, 1-MCP with or without ethylene resulted in reduced activity of chlorophyllase (Chlase) and peroxidase (POD). Exposure to C2H4 stimulated Chlase activity and extended the duration of high POD activity. Treatment with 1-MCP followed by C2H4 resulted in reduced POD activity and delayed the increase in Chlase activity. The results suggest chlorophyll in broccoli can be degraded via the POD-hydrogen peroxide system. Exposure to C2H4 enhances activity of Chlase and extends the duration of high POD activity, and these responses may accelerate degreening. Treatment with 1-MCP delays yellowing of broccoli, an effect that may be due to the 1-MCP-induced reduction in POD and Chlase activities.

(PDF emailed within 0-6 h: $19.90)

Accession: 003727902

Download citation: RISBibTeXText

DOI: 10.1023/a:1023058003002



Related references

Effect of ethylene and 1-methylcyclopropene on chlorophyll catabolism of broccoli florets. Plant Growth Regulation 40(1): 33-38, 2003

Chlorophyll and xanthophyll changes in broccoli florets stored under elevated CO2 or ethylene-containing atmosphere. Hortscience 33(1): 114-117, 1998

Effect of 1-methylcyclopropene on senescence and sugar metabolism in harvested broccoli florets. Postharvest Biology and Technology 116: 45-49, 2016

Wound-induced ethylene synthesis in stem tissue of harvested broccoli and its effect on senescence and ethylene synthesis in broccoli florets. Postharvest biology and technology 24(1): 69-78, 2002

Cytokinins affect respiration ethylene production and chlorophyll retention of packaged broccoli florets. Hortscience 25(1): 88-90, 1990

Effect of 1-methylcyclopropene on shelf life, visual quality, antioxidant enzymes and health-promoting compounds in broccoli florets. Food Chemistry8: 3, 774-781, 2010

The role of ethylene in the conservation and the regulation of the biosynthesis of ethylene of broccoli florets after harvest the effect of temperature. Canadian Institute of Food Science & Technology Journal 24(1-2): 42-46, 1991

The role of ethylene in storage and regulation of ethylene biosynthesis of broccoli florets after harvest. Effect of temperature. Canadian Institute of Food Science and Technology Journal 24(1-2): 42-47, 1991

Inhibition of Ethylene Biosynthesis and Perception by 1-Methylcyclopropene and Its Consequences on Chlorophyll Catabolism and Storage Quality of 'Bosc' Pears. Journal of the American Society for Horticultural Science 142(2): 92-100, 2017

Effect of 1-methylcyclopropene on expression of genes for ethylene biosynthesis enzymes and ethylene receptors in post-harvest broccoli. Plant Growth Regulation 57(3): 223-232, 2009

A novel pathway of chlorophyll catabolism in ethylene-treated broccoli (Brassica oleracea L.) flower buds. Bulletin of the Faculty of Agriculture, Miyazaki University 47(1/2): 51-57, 2000

Effect of Mg-dechelation activity on chlorophyll degradation in stored broccoli florets. Acta Horticulturae (712(Vol.2)): 705-709, 2006

Effect of sugars on ethylene synthesis and responsiveness in harvested broccoli florets. Postharvest Biology and Technology 36(2): 157-165, 2005

Involvement of Chlorophyll Degrading Enzymes with Chlorophyll Degradation in Stored Broccoli(Brassica oleracea L.) Florets. Food Science and Technology Research 5(3): 300-303, 1999

Impact of UV-B irradiation on chlorophyll degradation and chlorophyll-degrading enzyme activities in stored broccoli florets. Food Chemistry0: 3, 645-651, 2010