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Effect of leaf extract of Azadirachta indica on some phosphatases in Trichuris globulosa (Nematoda)



Effect of leaf extract of Azadirachta indica on some phosphatases in Trichuris globulosa (Nematoda)



Research Bulletin of the Panjab University, Science 52(1/4): 43-48



The effects of ethanolic neem leaf extract (prepared in Soxhlet apparatus) was studied on some phosphatases in Trichuris globulosa. Three enzymes, viz., glucose-6-phosphatase (G-6-pase), acid phosophatase (ACP) and alkaline phosphatase (ALP) were assayed in their respective buffers and these enzymes showed a typical Michaelis menten substrate saturation kinetics. Vmax (maximum of the apparent enzyme velocity) and Km (substrate affinity constant) were calculated directly from the plots.

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