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Effect of low temperature pretreatment of buds or inflorescence on isolated microspore culture in Brassica rapa (syn. B. campestris)

Effect of low temperature pretreatment of buds or inflorescence on isolated microspore culture in Brassica rapa (syn. B. campestris)

Breeding Science 52(1): 23-26

ISSN/ISBN: 1344-7610

DOI: 10.1270/jsbbs.52.23

The effect of low temperature pretreatment of buds or inflorescence on microspore culture for the production of haploids of B. rapa Tsubame (syn. B. campestris) was examined. Incubation of the buds or the inflorescence at 4 degrees C for 3 or 10 days before the culture of microspores induced efficient microspore embryogenesis. Pretreatment of flower buds was more effective than that of the inflorescence. Prolonged pretreatment up to 20 days promoted embryo induction.

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Accession: 003730779

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