EurekaMag
+ Translate
+ Most Popular
Advantages and disadvantages of bordeaux mixture and of lime-sulphur used on apples in the growing season
Observations on the Umaria marine bed
10 years of hearing conservation in the Royal Air Force
Chocolate crumb - dairy ingredient for milk chocolate
Effect of daily gelatin ingestion on human scalp hair
Comparison of rice bran and maize bran as feeds for growing and fattening pigs
The composition of pampas-grass (Cortaderia argentea.)
The Accraian Series:
The mechanism of the Liebermann-Burchard reaction of sterols and triterpenes and their esters
Cerebrovascular Doppler ultrasound studies (cv-Doppler)
Toria: PT-303 - first national variety
Hair growth promoting activity of tridax procumbens
Productivity of Pekin x Khaki Campbell ducks
A stable cytosolic expression of VH antibody fragment directed against PVY NIa protein in transgenic potato plant confers partial protection against the virus
Solar treatment of wheat loose smut
Swimmers itch in the Lake of Garda
Bactofugation and the Bactotherm process
The effects of prefrontal lobotomy on aggressive behavior in dogs
Visual rating scales for screening whorl-stage corn for resistance to fall armyworm
Breakdown of seamounts at the trench axis, viewed from gravity anomaly
Kooken; pennsylvania's toughest cave
Recovery of new dinosaur and other fossils from the Early Cretaceous Arundel Clay facies (Potomac Group) of central Maryland, U.S.A
Zubor horny (Bison bonasus) v prirodnych podmienkach Slovensku
The extended Widal test in the diagnosis of fevers due to Salmonella infection
Hair of the american mastodon indicates an adaptation to a semi aquatic habitat

Effect of maturation media and oocytes derived from sows or gilts on the development of cloned pig embryos


Effect of maturation media and oocytes derived from sows or gilts on the development of cloned pig embryos



Theriogenology 59(7): 1641-1649



ISSN/ISBN: 0093-691X

PMID: 12559468

DOI: 10.1016/s0093-691x(02)01211-6

In order to develop a culture system and recipient cytoplasm that could improve the developmental competence of somatic cell nuclear transfer (SCNT) embryos for successful cloning of pigs, we evaluated the effect of donor oocytes and in vitro maturation (IVM) media on maturation of oocytes and developmental competence of SCNT embryos. In Experiment 1, oocytes derived from sows or gilts were matured in two IVM media (TCM-199 versus NCSU-23) and maturation of oocytes was evaluated by the status of chromatin configuration, the diameter of matured oocytes, the thickness of the zona pellucida, and the size of the perivitelline space (PVS). Sow oocytes matured in TCM-199 (S-TCM group) and NCSU-23 (S-NCSU group) showed significantly higher (P<0.05) maturation rates (S-TCM and S-NSCU, 86+/-4 and 82+/-4%, respectively) when evaluated by metaphase-II status than the gilt oocytes matured in TCM-199 (G-TCM group, 71+/-3%) and in NCSU-23 (G-NCSU-23 group, 71+/-3%). Oocyte diameter, the thickness of the zona pellucida, and the perivitelline space of sow oocytes (S-TCM and S-NCSU) were larger than those of gilt oocytes (G-TCM and G-NCSU) after IVM (P<0.05). In Experiment 2, SCNT was performed, using in vitro-matured oocytes from each group as recipient cytoplasm and porcine fetal fibroblasts as karyoplasts. The reconstructed embryos were electrically fused and activated, and cleavage and blastocyst formation were monitored under a stereomicroscope. The total cell number of flattened blastocysts stained with 5 micromolar bisbenzimide on day 7 were counted. In addition, in vitro matured non-enucleated oocytes were also electrically activated (parthenogenetic activation) and pronuclear formation was monitored. No difference in pronuclear formation rate after parthenogenetic activation and fusion rate after SCNT was observed among experimental groups. A significantly higher cleavage rate (P<0.05) was observed in S-TCM (69+/-4%) when compared with only G-NCSU (58+/-4%), but not with G-TCM (60+/-4%) or S-NCSU (68+/-4%). The rate of blastocyst formation was significantly higher (P<0.05) in sow oocytes (24% in S-TCM and S-NCSU), when compared to that observed in G-TCM (15%), and G-NCSU (14%). When the same source of oocytes was used, there was no significant difference in rate of blastocyst formation in the two culture media. Total cell number of blastocysts were not significantly different among experimental groups. In conclusion, the present study clearly demonstrated that sow oocytes have a greater developmental competence than gilt oocytes, regardless of the maturation medium examined.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003730952

Download citation: RISBibTeXText

Related references

Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos. Veterinaria Mexico 5(4), 2018

Effects of leptin supplementation in in vitro maturation medium on meiotic maturation of oocytes and preimplantation development of parthenogenetic and cloned embryos in pigs. Animal Reproduction Science 101(1-2): 85-96, 2007

Effects of Some Factors on In Vitro Production of Embryos from Antral Follicle-Derived Porcine Oocytes Ⅰ. Effects of Maturation Media on In Vitro Maturation, Fertilization and Development. Journal of Animal Reproduciton and Biotechnology 19(2): 165-172, 2004

The development of intra- and inter-species cloned embryos derived from rabbit oocytes: the effect of donor cell sources. Thai Journal of Veterinary Medicine 37(1): 110, 2007

Effects of cumulus cells on in vitro maturation of oocytes and development of cloned embryos in the pig. Reproduction in Domestic Animals 47(4): 521-529, 2012

Lanosterol influences cytoplasmic maturation of pig oocytes in vitro and improves preimplantation development of cloned embryos. Theriogenology 85(4): 575-584, 2016

Duration of in vitro maturation of recipient oocytes affects blastocyst development of cloned porcine embryos. Cloning and Stem Cells 7(1): 35-44, 2005

The effect of gas phase on the in vitro development of bovine embryos derived from in vitro maturation and fertilization of ovarian oocytes. Theriogenology 37(1): 320, 1992

Effects of Some Factors on In Vitro Production of Embryos from Antral Follicle-Derived Porcine Oocytes Ⅱ. Effects of EGF and the Number of COCs into Maturation Media on In Vitro Maturation. Journal of Animal Reproduciton and Biotechnology 19(2): 173-183, 2004

In-vitro development of embryos derived from vitrified-warmed oocytes is delayed compared with embryos derived from fresh oocytes: a time-lapse sibling oocyte study. Reproductive Biomedicine Online 40(1): 82-90, 2020

Leptin promoted meiotic maturation of bovine oocytes and development of parthenogenetic activation and yak -bovine interspecies cloned embryos. Belgian Journal of Zoology 8(2): 184-190, 2008

Effect of different levels of energy in the ration on productivity and breeding capacity of gilts and sows. 5. Effect of different levels of energy in the ration of gilts on signs of oestrus, development and survival of embryos at the 21st and 40th days of pregnancy. Zbornik Radova Institut za Stocarstvo Novi Sad 5: 107-127, 1972

Development of in vitro-derived bovine embryos after maturation of oocytes in TCM-199 supplemented with EDTA and pyruvate. Theriogenology 39(1): 202, 1993

Development of bovine embryos derived from oocytes cultured in roscovitine for 24 or 48 hours before oocyte maturation. Biology of Reproduction 64(Suppl. 1): 141, 2001

Effect of different energy levels in food on production and reproductive capacity of gilts and sows. 5. Effect on oestrus manifestation, and development and survival of embryos to 21 and 40 days of gestation. Zbornik Radova, Institut za Stocarstvo, Novi Sad (5): 107-127, 1971