+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Effect of oligofructose or dietary calcium on repeated calcium and phosphorus balances, bone mineralization and trabecular structure in ovariectomized rats*



Effect of oligofructose or dietary calcium on repeated calcium and phosphorus balances, bone mineralization and trabecular structure in ovariectomized rats*



British Journal of Nutrition 88(4): 365-377



We investigated the effects of dietary oligofructose and Ca on bone structure in ovariectomized rats, using microradiography and histomorphometry. Ninety-six animals were allocated to seven experimental groups: G1, sham-operated; G2-G7, ovariectomized. Semi-purified diets containing 5 g Ca/kg (recommended content) without oligofructose (G1, G2) or with 25, 50 or 100 g oligofructose/kg (G3, G4, G5) or 10 g Ca/kg (high content) without oligofructose (G6) or with 50 g oligofructose/kg (G7) were fed for 16 weeks. At the recommended level of Ca, high oligofructose (G5) increased femur mineral levels in ovariectomized rats, while medium oligofructose did so at high Ca. Increasing Ca in the absence of oligofructose did not increase femur mineral content. Trabecular bone area (%) analysed in the tibia was 10.3 (sem 1.2) (G1), 7.7 (sem 0.6) (G2), 9.3 (sem 0.7) (G3), 9.4 (sem 1.0) (G4), 9.5 (sem 0.7) (G5), 10.2 (sem 0.8) (G6), and 12.6 (sem 0.8) (G7). At the recommended level of Ca, 25 g oligofructose/kg prevented loss of trabecular area due to increased trabecular thickness, while 50 or 100 g oligofructose/kg increased trabecular perimeter. At high Ca, oligofructose prevented loss of bone area due to increased trabecular number but similar thickness (G7 v. G6). When Ca was raised in the presence of oligofructose (G7), trabecular area and cortical thickness were highest, while loss of trabecular connectivity was lowest of all groups. At the same time, lumbar vertebra Ca was higher; 44.0 (sem 0.8) (G7) compared with 41.6 (sem 0.8) (G2), 41.4 (sem 0.7) (G4), and 40.5 (sem 1.0) mg (G6). We conclude that ovariectomy-induced loss of bone structure in the tibia was prevented but with different trabecular architecture, depending on whether dietary Ca was increased, oligofructose was incorporated, or both. Oligofructose was most effective when dietary Ca was high.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003731940

Download citation: RISBibTeXText

PMID: 12323086

DOI: 10.1079/bjn2002661


Related references

Effect of oligofructose on bone mineralization in ovariectomized rats is affected by dietary calcium. American Journal of Clinical Nutrition 73(2S): 498S, 2001

Effect of dietary calcium:phosphorus ratio on bone mineralization and intestinal calcium absorption in ovariectomized rats. BioFactors 22(1/4): 39-42, 2004

Effect of dietary calcium: Phosphorus ratio on bone mineralization and intestinal calcium absorption in ovariectomized rats. Biofactors 22(1-4): 39-42, 2004

Long term effect of oligofructose on bone trabecular structure in ovariectomized rats. American Journal of Clinical Nutrition 73(2S): 498S, 2001

Effect of dietary calcium vitamin c and protein in development of experimental skeletal fluorosis part 2 calcium turnover with calcium 45 calcium and phosphorus balances. Metabolism Clinical & Experimental 20(7): 650-656, 1971

The effect of dietary calcium phosphorus ratio on calcium metabolism in ovariectomized rats. Journal of Dental Research 60(SPEC ISSUE A): 553, 1981

Requirement for digestible calcium by 25 to 50 kg pigs at different dietary concentrations of phosphorus as indicated by growth performance, bone ash concentration, and calcium and phosphorus balances. Journal of Animal Science 94(12): 5272-5285, 2016

Effect of dietary calcium and phosphorus on mineral homeostasis, bone mineralization and calcium absorption in the neonatal pig model. Pediatric Research 33(4 Part 2): 304A, 1993

Effect of dietary calcium and phosphorus on mineral homeostasis, bone mineralization and calcium absorption in the neonatal swine model. FASEB Journal 7(3-4): A88, 1993

Effect of dietary calcium, vitamin C and protein in development of experimental skeletal fluorosis. II. Calcium turnover with 45Ca; calcium and phosphorus balances. Metabolism. 20: 7, 650-656, 1971

Dietary calcium chloride vs. calcium carbonate reduces urinary pH and phosphorus concentration, improves bone mineralization and depresses kidney calcium level in cats. Journal of Nutrition 124(11): 2212-2222, 1994

Reduction in dietary calcium/phosphorus ratio reduces bone mass and strength in ovariectomized rats enhancing bone turnover. Bioscience Biotechnology and Biochemistry 69(10): 1970-1973, 2005

Dietary calcium and phosphorus ratio regulates bone mineralization and turnover in vitamin D receptor knockout mice by affecting intestinal calcium and phosphorus absorption. Journal of Bone and Mineral Research 18(7): 1217-1226, 2003

Calcium metabolism and bone mineralization in female rats fed diets marginally sufficient in calcium: effects of increased dietary calcium intake. Bone and Mineral 12(1): 1-14, 1991

Dietary phosphorus depletion in sheep effects on plasma inorganic phosphorus calcium 1 25 dihydroxyvitamin d 3 and alkaline phosphatase and on gastrointestinal phosphorus and calcium balances. Journal of Agricultural Science 105(3): 623-630, 1985