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Enhanced detection of Prune dwarf virus in peach leaves by immunocapture-reverse transcription-polymerase chain reaction with nested polymerase chain reaction (IC-RT-PCR nested PCR)



Enhanced detection of Prune dwarf virus in peach leaves by immunocapture-reverse transcription-polymerase chain reaction with nested polymerase chain reaction (IC-RT-PCR nested PCR)



Journal of Phytopathology 150(2): 94-96



To improve the sensitivity of detection of Prune dwarf virus (PDV) in peach trees, the immunocapture-reverse transcription-polymerase chain reaction combined with nested PCR (IC-RT-PCR nested PCR) was modified. The nested PCR substantially improved PDV detection compared with double antibody sandwich-ELISA.

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Accession: 003751099

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DOI: 10.1046/j.1439-0434.2002.00696.x


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