Esterase isozymes are useful to track introgression between Allium fistulosum L. and A. cepa L

Hou, A.F.; Geoffriau, E.; Peffley, E.B.

Euphytica 122(1): 1-8


ISSN/ISBN: 0014-2336
DOI: 10.1023/a:1012679104502
Accession: 003754728

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Polyacrylamide gel electrophoresis was used to study the polymorphism of esterases in A. cepa (cultivars New Mexico Sunlite and TG1015) and A. fistulosum (cultivars Ishikura and Heshiko). Two varieties of each species, an A. fistulosum x A. cepa interspecific F1 hybrid (8273), and (A. fistulosum x A. cepa) hybrid derivatives were analysed for the presence of banding patterns upon staining with alpha - and beta -napthyl acetate substrates of esterase. Complex band patterns were observed. In total, 10 bands were detected between A. cepa and A. fistulosum - five in A. cepa, six in A. fistulosum with only one band common to both species. With the exception of one band unique to A. fistulosum which appeared only when stained with the alpha -substrate, extracts of both A. cepa and A. fistulosum leaf tissue exhibited the same bands when stained with both alpha - and beta -substrates. Bands stained with the different systems are distinguished by colour: the alpha -substrate always appeared black, while the bands stained with the beta -substrate are always red. Esterase bands were assigned into 5 presumptive loci of four zones of activity according to the migration distance of the bands from the front, colour of each band upon staining with alpha - and beta -substrates, and segregation observed in crosses and hybrid derivatives. Esterase enzymes detected in this study appear to be monomeric. Polymorphisms were identified between A. cepa and A. fistulosum by esterase banding patterns. Esterase enzymes provide an additional marker in monitoring introgression of foreign germplasm in interspecific onion breeding.