Evaluation of a novel method based on amplification of DNA fragments surrounding rare restriction sites (ADSRRS-fingerprinting) for typing bacterial strains

Krawczyk, B.; Lewandowski, K.; Kur, J.

Advances in Agricultural Sciences 8(1): 71-80

2002


Accession: 003757809

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Abstract
In the search for an effective DNA-typing technique for use in epidemiology, the performance and convenience of a novel assay based on the fingerprinting of bacterial genomes by amplification of DNA fragments surrounding rare restriction sites (ADSRRS-fingerprinting) was tested. A large number of vancomycin resistant E. faecium (VRE) and Acinetobacter isolates were examined. We found that ADSRRS-fingerprinting analysis is a rapid method that offers good discriminatory power. The method demonstrated also excellent reproducibility. The usefulness of the ADSRRS-fingerprinting method for molecular typing was compared with pulsed-field gel electrophoresis (PFGE) method, which is currently considered the gold standard for molecular typing of isolates recovered from patients and the environment in the course of investigation and control of nosocomial outbreaks. Clustering of ADSRRS-fingerprinting data matched pulsed-field gel electrophoresis data. The features of ADSRRS-fingerprinting technique is discussed in comparison with conventional methods.