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Expression of interferon-gamma and tumour necrosis factor-alpha in pigs experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV)



Expression of interferon-gamma and tumour necrosis factor-alpha in pigs experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV)



Journal of Comparative Pathology 127(2/3): 106-113



The expression of mRNA encoding interferon-gamma (IFN-gamma) and tumour necrosis factor-alpha (TNF-alpha) was studied, by reverse transcription-polymerase chain reaction and by in-situ hybridization with a non-radioactive digoxigenin-labelled probe, in formalin-fixed, paraffin wax-embedded lung tissue from pigs experimentally infected with a Korean isolate (North American genotype) of PRRSV. Both IFN-gamma and TNF-alpha mRNA were detected in the lung as early as 1 day post-inoculation (dpi) and up to 10 dpi. Hybridization signals for IFN-gamma and TNF-alpha were usually associated with inflammation, in particular with macrophages within alveolar septa. Expression of these cytokines was minimal in non-lesional lung of PRRSV-infected pigs and in normal lung from control pigs. The results suggest that IFN-gamma and TNF-alpha play a role in pulmonary defence against PRRSV.

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Accession: 003765492

Download citation: RISBibTeXText

PMID: 12354520

DOI: 10.1053/jcpa.2002.0566


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