+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Expression, purification, crystallization and preliminary crystallographic analysis of recombinant pteridine reductase of Trypanosoma cruzi



Expression, purification, crystallization and preliminary crystallographic analysis of recombinant pteridine reductase of Trypanosoma cruzi



Acta Crystallographica Section D, Biological Crystallography 57(11): 1671-1673



The recombinant version of Trypanosoma cruzi pteridine reductase was expressed in Escherichia coli, purified to homogeneity from the soluble fraction of bacterial extract by metal-chelate affinity chromatography and crystallized in the presence of the cofactor (NADPH) and an inhibitor (methotrexate) at 295 K using sodium acetate as precipitant. The crystals are trigonal, belonging to space group P3(1) (or P3(2)), with unit-cell parameters a = 74.35, c = 179.96 A under cryogenic conditions. The asymmetric unit contains a tetramer, with a corresponding V(M) of 2.3 A(3) Da(-1)and a solvent content of 46%. Native data have been collected to 2.1 A resolution using Cu Kalpha X-rays.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003765848

Download citation: RISBibTeXText

PMID: 11679740

DOI: 10.1107/s0907444901012094


Related references

Crystallization and preliminary crystallographic analysis of trypanothione reductase from Trypanosoma cruzi, the causative agent of Chagas' disease. FEBS Letters 317(1-2): 105-108, 1993

Expression, purification, crystallization and preliminary crystallographic analysis of Trypanosoma brucei phosphofructokinase. Acta Crystallographica Section D, Biological Crystallography 59(3): 532-534, 2003

Expression, crystallization and preliminary X-ray crystallographic analysis of glucose-6-phosphate dehydrogenase from the human pathogen Trypanosoma cruzi in complex with substrate. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 11): 1457-1461, 2011

Crystallization and preliminary crystallographic studies of dihydrofolate reductase-thymidylate synthase from Trypanosoma cruzi, the Chagas disease pathogen. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 65(Pt 11): 1175-1178, 2009

Superoxide reductase from Nanoarchaeum equitans: expression, purification, crystallization and preliminary X-ray crystallographic analysis. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 5): 591-595, 2011

Preparation, crystallization and preliminary crystallographic analysis of old yellow enzyme from Trypanosoma cruzi. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 63(Pt 10): 896-898, 2007

Expression, purification, crystallization and preliminary X-ray crystallographic analysis of a novel plant-type ferredoxin/thioredoxin reductase-like protein from Methanosarcina acetivorans. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 7): 775-778, 2011

Purification crystallization and preliminary crystallographic analysis of porcine aldose reductase. Journal of Molecular Biology 218(4): 695-698, 1991

Expression, purification, crystallization and preliminary X-ray crystallographic studies of Mycobacterium tuberculosis thioredoxin reductase. Acta Crystallographica. Section D Biological Crystallography 60(Pt 4): 777-779, 2004

Expression, purification, crystallization and preliminary X-ray crystallographic studies of Deinococcus radiodurans thioredoxin reductase. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications 62(Pt 8): 757-760, 2006

Purification, crystallization and preliminary X-ray crystallographic analysis of xylose reductase from Candida tropicalis. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 65(Pt 4): 419-421, 2009

Purification, crystallization and preliminary crystallographic analysis of a dissimilatory Sulfite Reductase DsrAB in complex with DsrC. 2008

Purification, crystallization and preliminary crystallographic analysis of a dissimilatory DsrAB sulfite reductase in complex with DsrC. Journal of Structural Biology 164(2): 236-239, 2008

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of GK0767, the copper-containing nitrite reductase from Geobacillus kaustophilus. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 6): 692-695, 2011

Expression, purification, crystallization and preliminary X-ray crystallographic studies of alkyl hydroperoxide reductase (AhpC) from the cyanobacterium Anabaena sp. PCC 7120. Acta Crystallographica. Section F Structural Biology and Crystallization Communications 67(Pt 10): 1203-1206, 2011