+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Genetic variability within Borrelia burgdorferi sensu lato genospecies established by PCR-single-strand conformation polymorphism analysis of the rrfA-rrlB intergenic spacer in Ixodes ricinus ticks from the Czech Republic



Genetic variability within Borrelia burgdorferi sensu lato genospecies established by PCR-single-strand conformation polymorphism analysis of the rrfA-rrlB intergenic spacer in Ixodes ricinus ticks from the Czech Republic



Applied and Environmental Microbiology 69(1): 509-516



In Europe the Borrelia burgdorferi sensu lato complex is represented by five distinct genospecies: Borrelia burgdorferi sensu stricto, Borrelia afzelii, Borrelia garinii, Borrelia valaisiana, and Borrelia lusitaniae. These taxonomic entities are known to differ in their specific associations with vertebrate hosts and to provoke distinct clinical manifestations in human patients. However, exceptions to these rules have often been observed, indicating that strains belonging to a single genospecies may be more heterogeneous than expected. It is, therefore, important to develop alternative identification tools which are able to distinguish Borrelia strains not only at the specific level but also at the intraspecific level. DNA from a sample of 370 Ixodes ricinus ticks collected in the Czech Republic was analyzed by PCR for the presence of a approximately 230-bp fragment of the rrfA-rrlB intergenic spacer of Borrelia spp. A total of 20.5% of the ticks were found to be positive. The infecting genospecies were identified by analyzing the amplified products by the restriction fragment length polymorphism (RFLP) method with restriction enzyme MseI and by single-strand conformation polymorphism (SSCP) analysis. The two methods were compared, and PCR-SSCP analysis appeared to be a valuable tool for rapid identification of spirochetes at the intraspecific level, particularly when large samples are examined. Furthermore, by using PCR-SSCP analysis we identified a previously unknown Borrelia genotype, genotype I-77, which would have gone unnoticed if RFLP analysis alone had been used.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003784421

Download citation: RISBibTeXText

PMID: 12514035

DOI: 10.1128/aem.69.1.509-516.2003


Related references

Prevalence of Borrelia burgdorferi sensu lato genospecies in host-seeking Ixodes ricinus ticks in selected South Bohemian locations (Czech Republic). Central European Journal of Public Health 12(3): 151-156, 2004

The occurrence of Ixodes ricinus ticks and important tick-borne pathogens in areas with high tick-borne encephalitis prevalence in different altitudinal levels of the Czech Republic Part II. Ixodes ricinus ticks and genospecies of Borrelia burgdorferi sensu lato complex. Epidemiologie, Mikrobiologie, Imunologie 65(3): 182-192, 2017

Genetic diversity of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from two areas in Slovakia and Czech Republic. FEMS Congress of European Microbiologists Abstract Book (1): 194, 2003

Occurrence of pathogenic genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus tick collected from north-western Poland Wystepowanie chorobotworczych genogatunkow z obrebu Borrelia burgdorferi sensu lato w kleszczach Ixodes ricinus odlawianych z Polnocno-Zachodniej Polski. Wiadomosci Parazytologiczne, 503: 545-553, 2004

Detection of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum in questing ticks Ixodes ricinus from the Czech Republic. Ticks and Tick-Borne Diseases 8(4): 483-487, 2017

Prevalence of Borrelia burgdorferi sensu lato in ticks, Ixodes ricinus in Tarnowskie Gory district Czestosc wystepowania Borrelia burgdorferi sensu lato u kleszczy Ixodes ricinus z okolic tarnowskich gor. Wiadomosci Parazytologiczne, 491: 39-45, 2003

Borrelia burgdorferi sensu lato genospecies in questing Ixodes ricinus ticks in Austria. International Journal Of Medical Microbiology8s1: Supplement 44, 168-176, 2008

Prevalence of Borrelia burgdorferi sensu lato genospecies in Ixodes ricinus ticks in Europe: a metaanalysis. Applied and Environmental Microbiology 71(11): 7203-7216, 2005

Simultaneous detection and genotyping of three genomic groups of Borrelia burgdorferi sensu lato in Dutch Ixodes ricinus ticks by characterization of the amplified intergenic spacer region between 5S and 23S rRNA genes. Journal of Clinical Microbiology 33(12): 3091-3095, 1995

Genetic diversity of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected in north-west Poland Genetyczna zmiennosc Borrelia burgdorferi s l u kleszczy Ixodes ricinus zebranych w polnocnozachodniej Polsce. Wiadomosci Parazytologiczne, 464: 475-485, 2000

Genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks from the Autonomous Province of Trento, Italy. International Journal of Medical Microbiology 297(1): 53-59, 2006

Detection of three genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected in different regions of Poland. International Journal of Medical Microbiology 290(6): 559-566, 2000

Diversity in prevalence and genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks and rodents in Lithuania and Norway. International Journal of Medical Microbiology 298(supp-S1): 0-187, 2008

Evaluation of occurrence of spirochetes Borrelia burgdorferi sensu lato in Ixodes ricinus ticks in selected areas of the Lublin region by polimerase chain reaction method PCR Ocena wystepowania kretkow Borrelia burgdorferi sensu lato w kleszczach Ixodes ricinus na terenie wybranych rejonow Lubelszczyzny przy zastosowaniu metody lancuchowej reakcji polimerazy PCR. Wiadomosci Parazytologiczne, 492: 165-171, 2003

Occurence of pathogenic genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from north-western Poland. Wiadomosci Parazytologiczne 50(3): 545-553, 2006