+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Genotypic, phenotypic, biochemical, physiological and pathogenicity-based categorisation of Acanthamoeba strains



Genotypic, phenotypic, biochemical, physiological and pathogenicity-based categorisation of Acanthamoeba strains



Folia Parasitologica 50(2): 97



The genus Acanthamoeba includes more than 20 morphological species, but classification is problematical. Recently, the discovery of substantial interstrain differences in ribosomal DNA (rDNA) sequences has prompted questions about the relatedness of strains of the same species. In this study, therefore, we have investigated relationships between two isolates of A. polyphaga, CCAP 1501/3c and ATCC 30871, using morphological, biochemical, physiological, molecular and cytotoxicity assays. We observed that A. polyphaga ATCC 30871 exhibited up to six arms in endocyst while A. polyphaga CCAP 1501/3c exhibited a maximum of 5 arms thus indicating their position in group 2 and 3, respectively. Acanthamoeba polyphaga ATCC 30871 exhibited growth at 37 degrees C and growth on 1M mannitol plates while A. polyphaga CCAP 1501/3c did not. In addition, both isolates exhibited differences in isoenzyme banding patterns and rDNA restriction fragment polymorphisms. More importantly, A. polyphaga ATCC 30871 produced cytotoxicity on corneal epithelial cells while A. polyphaga CCAP 1501/3c had no effects, suggesting differences in pathogenicity. Thus, all the results provide evidence for significant differences between the strains and further provided the basis for reclassification of the isolates. Implications of these results in the clinical diagnosis of pathogenic Acanthamoeba are discussed.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 003785802

Download citation: RISBibTeXText

PMID: 14560969


Related references

Genotypic, phenotypic, biochemical and physiological differentiation of pathogenic and non-pathogenic Acanthamoeba. Journal of Eukaryotic Microbiology 50(2): 24A-25A, March-April, 2003

Biochemical markers of pathogenicity and virulence of Acanthamoeba sp. strains. Parasitology Research 79(8): 696-698, 1993

Genotypic, physiological, and biochemical characterization of potentially pathogenic Acanthamoeba isolated from the environment in Cairo, Egypt. Parasitology Research 115(5): 1871-1881, 2016

Evaluation of practical usefulness of selected phenotypic and genotypic markers for pathogenicity of enterotoxic and entero-hemorrhagic strains of E.coli.. Medycyna Doswiadczalna i Mikrobiologia 54(2): 119-127, 2002

Genotypic diversity of Acanthamoeba strains isolated from Chilean patients with Acanthamoeba keratitis. Parasites and Vectors 12(1): 58, 2019

Phenotypic and genotypic analysis of pathogenicity of Yersinia pseudotuberculosis and Yersinia enterocolitica strains. Medycyna Weterynaryjna 58(4): 303-305, 2002

The phenomenon of heterosis and genotypic and phenotypic correlations among some physiological and biochemical characters in maize hybrids. Probleme de Genetica Teoretica si Aplicata 16(3): 167-185, 1984

Physiological and biochemical characteristics of Erwinia phytophthora strains differing in pathogenicity. Sel' skokhozyaistvennaya Biologiya (8): 109-111, 1986

Physiological biochemical peculiarities of erwinia phytophthora strains of varying pathogenicity. Sel'skokhozyaistvennaya Biologiya (8): 109-111, 1986

Pathogenicity in mice of strains of Acanthamoeba culbertsoni (Singh & Das, 1970) and A. rhysodes (Singh, 1952) from Indian soils and the present status of the genera Hartmannella and Acanthamoeba. Indian Journal of Parasitology 4(2): 135-144, 1980

Genotypic and phenotypic features of Arcobacter butzleri pathogenicity. Microbial Pathogenesis 76: 19-25, 2014

Comparative study of experimental pathogenicity of Acanthamoeba strains. Annales de Parasitologie Humaine et Comparee 55(5): 491-502, 1980

Mebendazole and levamisole influence on the pathogenicity of acanthamoeba polyphaga strains. Revista Iberica de Parasitologia 45(3): 249-257, 1985

Genotypic and phenotypic diversity in Enterococcus faecalis: is agar invasion a pathogenicity score?. Revista Espanola de Quimioterapia 28(2): 101-108, 2015

Isolation of two strains of Acanthamoeba castellanii from human tissue and their pathogenicity and isoenzyme profiles. Journal of Clinical Microbiology 18(6): 1405-1412, 1983