Germination studies on Atropa acuminata Royle ex Lindley

Muhammad Ibrar; Hussain, F.

Pakistan Journal of Botany 34(4): 341-344


ISSN/ISBN: 0556-3321
Accession: 003786539

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A. acuminata is an important medicinal plant containing tropane alkaloids that have anticholinergic, anodyne, antihistamine and mydriatic properties. This study was conducted to analyse A. acuminata germination and to apply different treatments to enhance germination. The treatments were: exposure to 15, 20, 25, 30 and 35 degrees C for 25 days; scarification through mechanical means, and exposure to 25 and 50%, and concentrated sulfuric acid for 75 seconds; scarified and unscarified seeds were soaked in water for 20 h and then immersed in hot water (60 degrees C) for 5 minutes; scarified and unscarified seeds were soaked in 1 ppm solutions of gibberellic acid (GA) and IAA for 20 h; intact and mechanically scarified seeds were exposed to concentrated sulfuric acid for 75 seconds, washed thoroughly and then soaked in water. Seeds that were mechanically scarified and then treated with IAA gave 78% germination while those subjected to acid scarification and soaking gave 50% germination. GA treatment resulted in 38% germination. All the other treatments were ineffective in inducing seed germination.