High-performance liquid chromatography determination of Zn-bacitracin in animal feed by post-column derivatization and fluorescence detection
Capitan-Vallvey, L.F.; Titos, A.; Checa, R.; Navas, N.
Journal of Chromatography A 943(2): 227-234
ISSN/ISBN: 0021-9673 PMID: 11833642 DOI: 10.1016/s0021-9673(01)01442-x
A sensitive and selective method is presented for the determination of Zn-Bacitracin in adulterated animal feed by reversed-phase ion-pair high-performance liquid chromatography and post-column derivatization with o-phthalaldehyde prior to fluorescence detection. The calibration function was estimated to be between 8.0 and 65.0 mg l(-1) of Zn-BC. The detection and quantification limits of the chromatographic method were 2.5 and 7.5 mg 1(-1), respectively. Using the extraction procedure of Zn-Bacitracin from the feedstuff that we recently proposed and applying this new chromatographic method, it was possible to detect this antibiotic at levels below 5 mg kg(-1) in different kinds of feedstuffs with a standard deviation less than 6.0%.