EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Immunisation of cattle against heartwater by infection with Cowdria ruminantium elicits T lymphocytes that recognise major antigenic proteins 1 and 2 of the agent


Immunisation of cattle against heartwater by infection with Cowdria ruminantium elicits T lymphocytes that recognise major antigenic proteins 1 and 2 of the agent



Veterinary immunology and immunopathology 85(1-2): 23-32



ISSN/ISBN: 0165-2427

PMID: 11867164

DOI: 10.1016/s0165-2427(01)00421-4

There is growing evidence that immunity of cattle to Cowdria ruminantium infection is mediated by T lymphocytes. C. ruminantium antigens that stimulate these responses are therefore of considerable importance to the development of a sub-unit vaccine against the disease. We have examined T cell responses against recombinant analogues of the surface-exposed C. ruminantium major antigen 1 (MAP1) a 28.8 kDa protein and MAP2 (21 kDa) antigen in cattle immunised by infection and treatment. Vigorous and sustained proliferative responses to both antigens were observed in peripheral blood mononuclear cells from immune cattle. MAP1-specific responses were predominantly restricted to cluster of differentiation four antigen positive T cells (CD4(+) T cells). Reverse transcription polymerase chain reaction (RT PCR) analysis of cytokine expression by T cell lines derived from this population revealed strong expression of interferon gamma (IFN-gamma), interferon alpha (IFN-alpha), tumour necrosis factor alpha (TNF-alpha), tumour necrosis factor beta (TNF-beta), interleukin-2 receptor alpha (IL-2Ralpha) transcripts, and weak expression of IL-2 and IL-4. Supernatants from these T cell cultures contained IFN-gamma protein. CD4(+) T cell clones specific for MAP1 were generated. Two of these clones proliferated in the presence of autologous infected endothelial cells. In contrast, the response to MAP2 was characterised largely by proliferation of gamma delta (gamma delta) T cells. RT PCR analysis of cytokine expression by T cell lines which were dominated by gamma delta T cells revealed expression of IFN-gamma, TNF-alpha, TNF-beta, IL-2Ralpha transcripts. Supernatants of these T cell cultures also contained IFN-gamma protein. Our findings indicate that immunisation of cattle by infection with C. ruminantium results in generation of MAP1- and MAP2-specific T cell responses that may play a role in protection against the pathogen.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 003802996

Download citation: RISBibTeXText

Related references

Immunization of cattle by infection with Cowdria ruminantium elicits T lymphocytes that recognize autologous, infected endothelial cells and monocytes. Infection and Immunity 66(5): 1855-1860, 1998

Major antigenic proteins 1 and 2 of Cowdria ruminantium are targets for T-lymphocyte responses of immune cattle. Annals of the New York Academy of Sciences 849: 372-374, 1998

Immunisation of sheep against heartwater with inactivated Cowdria ruminantium. Research in Veterinary Science 58(1): 46-49, 1995

Protection of goats against heartwater acquired by immunisation with inactivated elementary bodies of Cowdria ruminantium. Veterinary Immunology and Immunopathology 41(1-2): 153-163, 1994

Comparative efficacy of Freund's and Montanide ISA50 adjuvants for the immunisation of goats against heartwater with inactivated Cowdria ruminantium. Veterinary Parasitology 67(3-4): 175-184, 1996

Field experience with heartwater (Cowdria ruminantium) in cattle. Journal of the South African Veterinary Association 50(4): 323-325, 1979

Heartwater (Cowdria ruminantium infection) on São Tomé. Veterinary Quarterly 4(3): 106-107, 1982

Growth of Cowdria ruminantium, the causative agent of heartwater, in a tick cell line. Journal of Clinical Microbiology 38(3): 1238-1240, 2000

Demonstration of vertical transmission of Cowdria ruminantium, the causative agent of heartwater, from cows to their calves. Veterinary Parasitology 61(1-2): 119-132, 1996

Isolation of the causative agent of heartwater (Cowdria ruminantium) from three Amblyomma species in eight Districts of Kenya. Veterinary Record 140(1): 13-16, 1997

Improved culture conditions for Cowdria ruminantium (Rickettsiales), the agent of heartwater disease of domestic ruminants. Cytotechnology 4(3): 285-290, 1990

Heartwater (Cowdria ruminantium infection) as a cause of postrestocking mortality of goats in Mozambique. Clinical and Diagnostic Laboratory Immunology 8(4): 843-846, 2001

Isolation and transmission of Cowdria ruminantium (causal agent of heartwater disease) in Blue Nile Province, Sudan. Veterinary Research Communications 8(2): 141-145, 1984

DNA vaccination with map1 gene followed by protein boost augments protection against challenge with Cowdria ruminantium, the agent of heartwater. Vaccine 20(7-8): 1215-1225, 2002

The application of the indirect fluorescent antibody test to the serology of heartwater Cowdria ruminantium, sheep and cattle. Tick biology and control: proceedings of an International Conference January 27-29-1981 edited by GB Whitehead and JD Gibson: 2, 1981