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Immunohistochemistry of skin biopsies treated with proteinase K and microwave for diagnosis of persistent bovine viral diarrhoea virus infection


Immunohistochemistry of skin biopsies treated with proteinase K and microwave for diagnosis of persistent bovine viral diarrhoea virus infection



Archivos de Medicina Veterinaria 35(1): 23-36



The aims of this study were to set up and to evaluate an immunohistochemical technique in bovine skin biopsies for the diagnosis of cattle persistently infected with bovine viral diarrhoea virus (BVDV). Formalin fixed, paraffin-embedded skin tissues were submitted to different treatments for antigen retrieval, incubated with 15.c.5, WB160, WB162, WB210 and WB215 monoclonal antibodies and developed with the biotin-streptoavidin-peroxidase system.

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Accession: 003803467

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