+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Improved cultural detection of Burkholderia cepacia from sputum in patients with cystic fibrosis



Improved cultural detection of Burkholderia cepacia from sputum in patients with cystic fibrosis



Journal of Clinical Pathology 54(10): 803-805



Aims: To evaluate the sensitivity and specificity of 2 selective media for the isolation of Burkholderia cepacia from sputum specimens in patients with cystic fibrosis (CF) in the UK Methods: 149 expectorated sputum specimens from 113 patients with CF (32 B. cepacia-colonized patients and 81 non-B. cepacia-colonized patients) attending 3 CF centres were examined for the presence of B. cepacia on 2 selective media: (1) MAST selective agar, a commercially available selective medium widely used in the UK and (2) BCSA (B. cepacia selective agar), a new medium recently described, which is used predominantly in North America. Results: B. cepacia was isolated from 53 of 149 (35.6%) specimens examined, representing 32 of 113 (28.3%) patients, using both the MAST and BCSA media. Growth was most rapid on BCSA with all (53 of 53) isolates detectable after 48 h, compared with 50 of the 53 isolates on MAST agar, with the remaining 3 isolates detectable at 5 days. 28 contaminants were identified on MAST agar and 13 on BCSA agar; mainly Alcaligenes xylosoxidans and yeast on MAST agar and Flavobacterium indologenes on BCSA medium. BCSA was equivalent to MAST agar in its ability to isolate B. cepacia from patients with CF with a history of B. cepacia infection. Conclusions: The increased selectivity and reduced time to detection of BCSA makes it an attractive alternative to MAST.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 003806050

Download citation: RISBibTeXText

PMID: 11577134


Related references

Improved molecular detection of Burkholderia cepacia genomovar III and Burkholderia multivorans directly from sputum of patients with cystic fibrosis. Journal of Microbiological Methods 49(2): 183-191, 2002

Comparison of culture and PCR for detection of Burkholderia cepacia in sputum samples of patients with cystic fibrosis. Journal of Clinical Microbiology 36(6): 1642-1645, 1998

PCR as a diagnostic tool for the rapid detection of Burkholderia cepacia in the sputum of patients with cystic fibrosis. Pediatric Research 43(4 Part 2): 336A, 1998

PCR-based detection and identification of Burkholderia cepacia complex pathogens in sputum from cystic fibrosis patients. Journal of Clinical Microbiology 39(12): 4247-4255, 2001

Polymerase chain reaction for the detection of Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Burkholderia cepacia in sputum of patients with cystic fibrosis. Molecular and Cellular Probes 10(6): 397-403, 1996

Burkholderia cepacia selective agar can be useful for recovery of Exophiala dermatitidis from sputum samples of cystic fibrosis patients. Journal of Cystic Fibrosis 15(2): E19, 2016

Direct culture-independent Strain typing of Burkholderia cepacia complex in sputum samples from patients with cystic fibrosis. Journal of Clinical Microbiology 48(5): 1888-1891, 2010

Epidemiology of pulmonary colonization with Burkholderia cepacia in cystic fibrosis patients. The French Observatoire Burkholderia cepacia Study Group. European Journal of Clinical Microbiology and Infectious Diseases 15(10): 841-842, 1996

Antibody response to Burkholderia cepacia in patients with cystic fibrosis colonized with Burkholderia cepacia and Pseudomonas aeruginosa. Journal of Infection 40(2): 164-170, 2000

Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients. International Journal of Systematic and Evolutionary Microbiology 51(Pt 2): 271-279, 2001

Accurate identification of members of the Burkholderia cepacia complex in cystic fibrosis sputum. Letters in Applied Microbiology 62(3): 221-229, 2016

Misidentification of Burkholderia cepacia in US cystic fibrosis treatment centers: an analysis of 1,051 recent sputum isolates. Chest 117(6): 1661-1665, 2000

Utility of commercial systems for identification of Burkholderia cepacia complex from cystic fibrosis sputum culture. Journal of Clinical Microbiology 38(8): 3112-3115, 2000

Comparison of Burkholderia cepacia Selective Agar versus Pseudomonas cepacia agar for the recovery of Burkholderia cepacia from 818 respiratory specimens from individuals with cystic fibrosis. Abstracts of the General Meeting of the American Society for Microbiology 101: 231, 2001

Pulmonary function, serum markers of inflammation, and IgG antibodies to core lipopolysaccharide of Burkholderia cepacia in adults with cystic fibrosis, following colonization with Burkholderia cepacia. Pediatric Pulmonology 29(1): 8, 2000