In vitro propagation of mature Japanese apricot (Prunus mume Sieb. et Zucc.)

Yonemitsu, H.; Nishi, K.; Sagan, S.; Tong, L.; Matsumura, Y.

Horticultural Research Japan 2(2): 77-82


DOI: 10.2503/hrj.2.77
Accession: 003808381

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Winter buds taken from mature trees of Japanese apricot (Prunus mume) were successfully established in culture on woody plant (WP) agar medium supplemented with 4.4 micro M benzyladenine (BA) and 3% glucose. The efficiencies of development of the bud into normal shoots are 50 to 100%. The efficiencies were improved by treating the buds with antioxidants, ascorbic acid, reduced glutathione, ferulic acid, and caffeic acid. Shoot proliferation in subcultures was also best in WP agar medium containing 4.4 micro M BA and 3% glucose. Root formation was induced when shoots were transferred into WP agar medium containing 0.54 micro M NAA. Rooted plantlets were incubated on autoclaved vermiculite containing fertilizer, and growing plantlets were then transferred to soil and successfully acclimated.