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In vivo administration of glucosamine inhibited phosphatidylinositol 3-kinase activity without affecting tyrosine phosphorylation of the insulin receptor or insulin receptor substrate in rat adipocytes

Miki, T.; Sakaue, M.; Kasuga, M.

Kobe Journal of Medical Sciences 48(3-4): 105-114

2002

ISSN/ISBN: 0023-2513
PMID: 12502902
Accession: 003808725
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We examined insulin signalling in rat epididymal adipocytes which developed insulin resistance by the in vivo infusion of glucosamine. Insulin-stimulated 2-deoxyglucose uptake into the adipocytes isolated from rats which were infused glucosamine for 4 h was diminished by 26%. To analyse insulin signalling in adipocytes, the epididymal fat tissues were harvested 5 min after insulin administration (10 U/kg), which was administered immediately after glucosamine infusion.

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