In vivo administration of glucosamine inhibited phosphatidylinositol 3-kinase activity without affecting tyrosine phosphorylation of the insulin receptor or insulin receptor substrate in rat adipocytes
Miki, T.; Sakaue, M.; Kasuga, M.
Kobe Journal of Medical Sciences 48(3-4): 105-114
We examined insulin signalling in rat epididymal adipocytes which developed insulin resistance by the in vivo infusion of glucosamine. Insulin-stimulated 2-deoxyglucose uptake into the adipocytes isolated from rats which were infused glucosamine for 4 h was diminished by 26%. To analyse insulin signalling in adipocytes, the epididymal fat tissues were harvested 5 min after insulin administration (10 U/kg), which was administered immediately after glucosamine infusion.