Induction of gynogenesis in muskellunge with irradiated sperm of yellow perch proves diploid muskellunge male homogamety
Dabrowski, K.; Rinchard, J.; Lin, F.; Garcia-Abiado, M.A.; Schmidt, D.
Journal of Experimental Zoology 287(1): 96-105
2000
ISSN/ISBN: 0022-104X
PMID: 10861556
DOI: 10.1002/1097-010x(20000615)287:1<96::aid-jez13>3.0.co;2-n
Accession: 003811448
Diploid gynogenesis was induced in muskellunge Esox masquinongy using UV-irradiated muskellunge sperm as the first step in producing monosex females. In this approach, we have to rely on negative controls as an indirect reference for sperm genetic material destruction. In the first experiment, equal proportions of gynogenetic females and males were produced. Negative controls, UV-irradiated sperm without heat shock, yielded some normal hatching larvae, described as spontaneous diploids.