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Interaction between root and callus of Pinus densiflora and an ectomycorrhizal fungus, Tricholoma matsutake



Interaction between root and callus of Pinus densiflora and an ectomycorrhizal fungus, Tricholoma matsutake



Mycosystema 22(3): 369-373



A simple in vitro system is described for interaction between tissues of P. densiflora and an ectomycorrhiza fungus, T. matsutake. The callus and roots, which were initiated from hypocotyl segments, were inoculated with T. matsutake under artificial conditions. After a 2-week incubation, fungal hyphae colonized the root surface. Fungal hyphae occurred within the root cortex, and Hartig net was formed after 3 weeks.

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