Measurement of logarithmic phase in Leptospira interrogans serovar icterohaemorrhagiae strain Lai
Huang HongLei; Xu JianGuo; Jiang XiuGao et al.
Chinese Journal of Zoonoses 18(3): 60-63
2002
Accession: 003842316
A study was conducted to investigate the growth characteristics of Leptospira for the proteome analysis of the organism. Leptospira was incubated in EMJH medium using a shaker with a vibration frequency of 90 rpm. The pH of the medium was controlled at 7.4 and the temperature at 30 degrees C. The viable count and OD600 of Leptospira over time was continually measured.
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